2 × SYBR yegirini qPCR MIX (NeYakakwira ROX)

Product features

Ichi chigadzirwa, 2 × SYBR girini qPCR MIX, chinouya muchubhu imwe ine zvese zvinodikanwa kuti PCR ikwidziridze uye kuona, kusanganisira Taq DNA poLymerase, SYBR yegirini I dhayi, High ROX Reference Dye, dNTPs, Mg2+, uye PCR buffer.
SYBR yegirini I dhayi idhayi regirinhi refluorescent rinosunga kuDNA ine tambo mbiri (double-strand DNA, dsDNA) kaviri helix minor groove region.SYBR yegirini I fluoresces isina simba munzvimbo yakasununguka, asi kana ichinge yasunga kuviri-tambo DNA. , fluorescence yayo inowedzerwa zvakanyanya. Izvi zvinoita kuti zvikwanise kuverenga huwandu hwekaviri-yakasungwa DNA yakagadzirwa panguva yePCR kukwidziridzwa nekuona iyo fluorescence intensity.
ROX inoshandiswa sedhayi yekururamisa kugadzirisa fluorescence kushanduka kusina hukama nePCR, nekudaro kuderedza misiyano yenzvimbo. Misiyano yakadaro inogona kukonzerwa nemhando dzakasiyana-siyana, senge pipette kukanganisa kana sampuli evaporation. Yakasiyana fluorescence quantification zviridzwa zvine zvakasiyana zvinodiwa zveROX, uye chigadzirwa ichi chakakodzera fluorescence quantification analyzer inoda High ROX kururamiswa.