2 × SYBR green qPCR MUX （NeHigh ROX）

Zvimiro zvechigadzirwa

Chigadzirwa ichi, 2×SYBR green qPCR MIX, chinouya muchubhu imwe chete ine zvese zvinodiwa pakuwedzera uye kuona PCR, zvinosanganisira Taq DNA poLymerase, SYBR green I dye, High ROX Reference Dye, dNTPs, Mg2+, uye PCR buffer.
Dhayi reSYBR green I idhayi regirini rinopenya rinobatana neDNA ine tambo mbiri (double-strand DNA, dsDNA) double helix minor groove region. SYBR green I inopenya zvishoma mu free state, asi kana yabatana neDNA ine tambo mbiri, fluorescence yayo inowedzera zvakanyanya. Izvi zvinoita kuti zvikwanisike kuverenga huwandu hweDNA ine tambo mbiri inogadzirwa panguva yePCR amplification nekuona kusimba kwefluorescence.
ROX inoshandiswa sedhayi yekugadzirisa kushanduka kwechiedza chechiedza chechiedza (fluorescence) kusina chekuita nePCR, nokudaro ichideredza kusiyana kwenzvimbo. Kusiyana kwakadaro kunogona kukonzerwa nezvinhu zvakasiyana-siyana, zvakaita sekukanganisa kwepipette kana evaporation yemuenzaniso. Zvishandiso zvakasiyana zve fluorescence quantification zvine zvinodiwa zvakasiyana zveROX, uye chigadzirwa ichi chakakodzera fluorescence quantification analyzers dzinoda High ROX correction.