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Kiti yekuchenesa DNA yeMagaPure yeMhuka yeMhuka yeGenomic yeMagaPure
Sumo pfupi
Kit iyi inoshandisa sisitimu ye "buffer system" yakagadzirwa uye yakagadziriswa uye mabhedhi emagineti anobatana zvakananga neDNA. Inogona kusunga, kunyudza, kuparadzanisa, uye kuchenesa nucleic acids nekukurumidza, uye yakagadzirirwa mhuka dzemumvura. Yakakodzera zvakanyanya kuburitsa nekuchenesa genomic DNA kubva munyama dzakasiyana dzemumvura, uye inogona kubvisa tsvina dzakadai semapuroteni, mafuta, nezvimwe zvinhu zve organic kusvika padanho rakakwana. Nekutsigira kushandiswa kweBigfish Magnetic Bead Nucleic Acid Extractor, yakakodzera kwazvo kuburitsa otomatiki saizi huru dzesamples. Genomic DNA yakabviswa ine kuchena kwakanyanya uye mhando yakanaka, uye inogona kushandiswa zvakanyanya muPCR/qPCR, NGS, Southern hybridization nedzimwe tsvakiridzo dzekuedza.
Zvimiro zvechigadzirwa
◆ Mienzaniso inoshanda zvakanyanya: DNA yeGenomic inogona kutorwa zvakananga kubva mumienzaniso yakasiyana-siyana yemhuka dzemumvura
◆ Yakachengeteka uye haina chepfu: Reagent haina zvinonyungudutsa zvine chepfu zvakaita sephenol nechloroform, ine simba rakawanda rekuchengetedza.
◆ Otomatiki: Yakashongedzerwa neBigfish Nucleic Acid Extractor, inogona kuburitsa zvinhu zvakawanda, kunyanya zvakakodzera kuburitsa zvinhu zvakakura.
◆ Kuchena kwakanyanya: kunogona kushandiswa zvakananga pakuedza kwemakemikari emuviri senge PCR, kugaya ma enzyme, kusanganisa, nezvimwewo.
Maitiro ekubvisa
Kutora Sample: Tora 25-30 mg yenyama yemhuka yemumvura
Kukuya: kukuya nenitrogen yemvura, kukuya kana kugerwa
Kugaya: 56℃ kugaya kwemvura inodziya mukugeza
Pamuchina: Isa supernatant pakati pema "centrifuge" woiisa mundiro kuti ibudiswe.
Magadzirirwo ehunyanzvi
Muenzaniso we sage: 25-30mg
Kuchena kweDNA: A260/280≧1.75
Chishandiso chinochinjika
Hove hombe BFEX-32/BFEX-32E/BFEX-96E
Tsanangudzo yechigadzirwa
| Zita rechigadzirwa | Katsi. Aihwa. | Kurongedza |
| MagaYakachenaMhuka dzemumvura GenomicKiti Yekuchenesa DNA(pkuzadza pasuru zvakare) | BFMP21R | 32T |
| MagaYakachenaMhuka dzemumvura GenomicKiti yekuchenesa DNA (pakeji yakazadzwa kare) | BFMP21R1 | 40T |
| MagaYakachenaMhuka dzemumvura GenomicKiti yekuchenesa DNA (pakeji yakazadzwa kare) | BFMP21R96 | 96T |
| RNase A(pchinhu chakatengwa) | BFRD017 | 1ml/chubhu (10mg/ml) |
Zvirongwa zvekuchengetedza ruzivo