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Nzira yeMagnetic Bead Inogadzirisa Matambudziko Mukubvisa DNA yeMvura Zvakatipoteredza
Munzvimbo dzakadai seongororo yezvakatipoteredza zve microbiology uye kutarisa kusvibiswa kwemvura, kuburitswa kweDNA re genomic remhando yepamusoro chinhu chakakosha pakushandiswa kwemishonga inotevera kusanganisira PCR/qPCR uye next-generation sequencing (NGS). Zvisinei, mienzaniso yemvura yezvakatipoteredza yakaoma zvikuru, ine nharaunda dzakasiyana dzehutachiona, mhando dzakaoma dzakadai seGram-positive bacteria, uye matambudziko enguva refu ane chekuita nenzira dzechinyakare dzekubvisa - dzakadai sekushandisa ma reagents ane chepfu uye maitiro akaomarara - izvo zvagara zvichinetsa vaongorori.
Iye zvino, Bigfish Sequencing yaunza BFMP24R Magnetic Bead-Based Environmental Water Genomic DNA Extraction and Purification Kit, iyo inopa mhinduro yakakwana kumatambudziko aya kuburikidza netekinoroji itsva uye dhizaini iri nyore kushandisa.
Pfupiso yeChigadzirwa
Kit iyi yakavakirwa pahurongwa hwe "buffer system" hwakagadziriswa pamwe chete nema "nano magnetic beads" anoshanda zvakanyanya. Genomic DNA inonyatsobatana nemapoka anoshanda ari pamusoro pema "beads" uye inoparadzaniswa pasi pesimba remagineti rekunze. Mushure mematanho akawanda ekusuka zvinyoro nyoro kubvisa mapuroteni, munyu, nezvimwe tsvina, genomic DNA inozopedzisira yadzimwa.
Yakagadzirirwa chaizvo mienzaniso yemvura yezvakatipoteredza, kit iyi inobvisa DNA yebhakitiriya inounganidzwa pama filter membranes zvinobudirira, kusanganisira mabhakitiriya eGram-negative neGram-positive (anosvika 2 × 10⁹ bacterial cells pa filter membrane imwe chete). Inoenderana nema system ekubvisa nucleic acid otomatiki ekugadzirisa zvinhu zvakawanda. DNA inobviswa ine mhando dzakafanana uye inogona kushandiswa zvakananga paPCR/qPCR, NGS, nedzimwe nzira dziri pasi.
Zvimiro zveChigadzirwa
1. Kugona Kubvisa Mabhakitiriya Akawanda
Inobvisa mabhakitiriya eGram-negative neGram-positive kubva mumvura zvinobudirira, ichifukidza nharaunda dzemabhakitiriya anowanikwa mumvura yakachena uye yegungwa, uye ichizadzisa zvinodiwa zvakasiyana-siyana zvekuongorora.
2. Kuchena Kwakanyanya uye Goho Rakawanda
Inopa DNA yakachena kwazvo, isina zvinosvibisa zvinodzivirira, uye goho rakasimba rakakodzera kushandiswa zvakananga mumamorekuru.
3. Kuenderana otomatiki uye Kushanda Kwazvo
Inoshanda zvizere neBigfish automated nucleic acid extraction systems, ichitsigira kugadziriswa kwemasampuli makumi matatu nemaviri kana makumi mapfumbamwe nematanhatu panguva imwe chete, zvichideredza zvakanyanya nguva yekugadzirisa uye kuvandudza kushanda zvakanaka kwerabhoritari.
4. Kushanda Kwakachengeteka uye Kunoshanda Nevashandisi
Hapana chikonzero chekushandisa zvinhu zvine chepfu zvakaita sephenol kana chloroform, zvichideredza njodzi dzekuchengetedzwa kwemurabhoritari. Zvinhu zvine chepfu zvinoiswa mumapuleti ane matsime makumi mapfumbamwe nematanhatu, zvichideredza zvikanganiso zvekushandisa pakuisa mapaipi nemaoko uye zvichiita kuti mashandiro ebasa ave nyore.
Zvishandiso Zvinoenderana
Hove hombe BFEX-16E
BFEX-32
BFEX-32E
BFEX-96E
Mhedzisiro Yekuyedza
Sampuro yemvura yerwizi ye600 mL yakaseferwa nepa membrane, uye DNA yakatorwa uchishandisa Bigfish magnetic bead-based environmental water genomic DNA extraction and purification kit pamwe chete nechishandiso chinoenderana nacho. DNA yakabviswa yakazoongororwa ne agarose gel electrophoresis.
M: Mark1, 2: Mienzaniso yemvura yerwizi
Zvimiro zveChigadzirwa
Nguva yekutumira: Zvita-18-2025
Zvirongwa zvekuchengetedza ruzivo