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Muminda yekuongororwa kwekiriniki mu vitro (IVD), genotyping, uye ongororo yemamorekuru, mienzaniso yemuromo—yakadai semishonga yekubvisa muromo, mishonga yekubvisa pahuro, uye mate—anoshandiswa zvakanyanya pakuongorora nucleic acid nekuda kwekuunganidzwa kuri nyore, hunhu husingakuvadzi, uye maitiro ekutora sampuli asingarwadziZvisinei, mienzaniso yemuromo inowanzova nehuwandu hushoma hwe nucleic acidsuye dzinowanzo svibiswa nemapuroteni nezvimwe zvinhu zvisina kuchenaNzira dzechinyakare dzekubvisa zvirimwa dzinowanzo kanganiswa nekushandisamashandiro akaomarara, kushanda zvishoma, uye kushandiswa kwezvinokonzera chepfu, izvo zvinogona kukanganisa zvikuru kururama nekugadzikana kwemashandisirwo ari pasi pemvura akadai sePCR/qPCR uye kutevedzana kwechizvarwa chinotevera (NGS).
IyoBFMP06 Magnetic Bead-Based Oral Swab Genomic DNA Extraction Kit, yakagadzirwa naHangzhou Bigfish FeiXu Biotechnology, inopamhinduro yakachengeteka, inoshanda, uye yakavimbikayekubvisa DNA yemuenzaniso wemuromo. Nekugadzirwa kwayo kwehunyanzvi uye mitemo yakasimba yekushanda, kit iyi yave chishandiso chinovimbwa nacho mumarabhoritari ekiriniki neekutsvagisa.
Kiti yeBFMP06 yakavakirwa pasisitimu yebuffer yakagadziriswa zvakanaka kwazvozvakabatanidzwa neMabhiza emagineti ehydroxyl chaiwo eDNA, zvichigadzira nzira inoshanda zvikuru yekuchenesa nucleic acid. Mushure mekunge sampuro yaiswa mu lysis buffer, zvikamu zvemasero zvinokanganiswa uye nucleic acids dzinoburitswa. Mapoka anoshanda ari pamusoro pemagineti anosunga DNA yakasununguka, zvichiita kuti ive yakagadzikana.magineti bead–DNA complexes.
Pasi pesimba remagineti rekunze, macomplexes anonyuramatanho maviri chaiwo ekugezakubvisa mapuroteni, munyu, nezvimwe zvinosvibisa. Chekupedzisira,DNA yemajini yakachena kwazvoinobviswa zvinobudirira uchishandisa olution buffer.
Tsananguro yeChigadzirwa
Chigadzirwa ichi chinoshandisa sisitimu yekudzivirira yakagadzirwa uye yakagadziriswa pamwe chetemabhora emagineti anobatanidza DNA zvakananga, zvichiita kuti ikwanise kunyudzwa, kuparadzaniswa, uye kucheneswa kwe nucleic acids nekukurumidza. Yakakodzera zvikuruKubviswa kweDNA ye genomic nekukurumidza uye zvinobudirira kubva muzvinwiwa zvinotorwa nemuromo, zvinwiwa zvinotorwa pahuro, uye sampuli dzemate, ukuwo ichibvisa mapuroteni nemunyu zvinobudirira.
Kana ikashandiswa pamwe chete neZviridzwa zvekuburitsa nucleic acid zveBigfish FeiXu zvine magnetic bead-based nucleic acid, kit iyi yakanakirakuburitsa otomatiki kwemagetsi akawandaDNA ye genomic yakacheneswa ndeyekuchena kwakanyanya uye mhando yepamusoro, zvichiita kuti ive yakakodzera kune akasiyana-siyana mashandisirwo ari pasi, kusanganisiraPCR/qPCR uye NGS.
Zvimiro zveChigadzirwa
Hunhu Hwepamusoro
Inoparadzanisa uye inochenesa DNA yemajini kubva mumasero emuviri zvinobudiriramishonga yekubvisa muromo, mishonga yekubvisa pahuro, uye mate, kuunzagoho guru uye kuchena kwakanyanya.
Kukurumidza uye Zviri nyore
Hapana matanho ekudzokorora kutenderera kana kupfapfaidza vacuum anodiwa. Inoenderana nezvishandiso zvekuburitsa otomatiki, zvichiita kuti ive yakakodzera kunekugadzirisa sampuro kwakakura.
Yakachengeteka uye Haina Chepfu
Hapana chikonzero chekushandisa zvinhu zvine chepfu zvakaita sephenol kana chloroform.
Zvishandiso Zvinoenderana
Hove hombe FeiXu BFEX-16E
BFEX-32
BFEX-32E
BFEX-96
Mhedzisiro Yekuyedza
Sampuli dzemushonga wemuromo (dzakanyikwa muMhinduro yekuchengetedza ye400 μL) uye sampuro dzemate (200 μL mate + 200 μL mushonga wekuchengetedza) dzakagadziriswa uchishandisaBigfish FeiXu Oral Swab Genomic DNA Purification KitDNA yakanyangarika mu70 μL yekugadziriswa kwemvurauye yakaongororwa naelectrophoresis ye gel agarose, sezvakaratidzwa mumufananidzo uri pazasi.
M: chiratidzo cheDNA (2K Plus II)
Zvimiro zveChigadzirwa
Nguva yekutumira: Ndira-15-2026
Zvirongwa zvekuchengetedza ruzivo