Bigfish Oral Swab DNA Purification Kit: Mhinduro Inoshanda Zvikuru Yekubvisa DNA Mumuromo

Muminda yekuongororwa kwekiriniki mu vitro (IVD), genotyping, uye ongororo yemamorekuru, mienzaniso yemuromo—yakadai sezvidziyo zvemuromo, zvidziyo zvepahuro, uye mate—anoshandiswa zvakanyanya pakuongorora nucleic acid nekuda kwekuunganidzwa kuri nyore, hunhu husingakuvadzi, uye maitiro ekutora sampuli asingarwadziZvisinei, mienzaniso yemuromo inowanzova nehuwandu hushoma hwe nucleic acidsuye dzinowanzo svibiswa nemapuroteni nezvimwe zvinhu zvisina kuchenaNzira dzechinyakare dzekubvisa zvirimwa dzinowanzo kanganiswa nekushandisamashandiro akaomarara, kushanda zvishoma, uye kushandiswa kwezvinokonzera chepfu, izvo zvinogona kukanganisa zvikuru kururama nekugadzikana kwemashandisirwo ari pasi pemvura akadai sePCR/qPCR uye kutevedzana kwechizvarwa chinotevera (NGS).

IyoBFMP06 Magnetic Bead-Based Oral Swab Genomic DNA Extraction Kit, yakagadzirwa naTekinoroji yeBiotechnology yeHangzhou Bigfish, inopamhinduro yakachengeteka, inoshanda, uye yakavimbikayekubvisa DNA yemuenzaniso wemuromo. Nekugadzirwa kwayo kwehunyanzvi uye mitemo yakasimba yekushanda, kit iyi yave chishandiso chinovimbwa nacho mumarabhoritari ekiriniki neekutsvagisa.

Kiti yeBFMP06 yakavakirwa pasisitimu yebuffer yakagadziriswa zvakanaka kwazvozvakabatanidzwa neMabhiza emagineti ehydroxyl chaiwo eDNA, zvichigadzira nzira inoshanda zvikuru yekuchenesa nucleic acid. Mushure mekunge sampuro yaiswa mu lysis buffer, zvikamu zvemasero zvinokanganiswa uye nucleic acids dzinoburitswa. Mapoka anoshanda ari pamusoro pemagineti anosunga DNA yakasununguka, zvichiita kuti ive yakagadzikana.magineti bead–DNA complexes.

Pasi pesimba remagineti rekunze, macomplexes anonyuramatanho maviri chaiwo ekugezakubvisa mapuroteni, munyu, nezvimwe zvinosvibisa. Chekupedzisira,DNA yemajini yakachena kwazvoinobviswa zvinobudirira uchishandisa olution buffer.

Tsananguro yeChigadzirwa