Nhau - Canine Multidrug Resistance: Maitiro eNucleic Acid Testing Inobatsira Kugonesa "Chaiyo Hazard Kuonekwa"

Dzimwe imbwa dzinotora antiparasite mishonga pasina nyaya, asi dzimwe dzichikurakurutsa uye manyoka. Iwe unogona kupa imbwa yako painkiller zvichienderana nehuremu hwayo, asi inogona kunge isina mhedzisiro kana inosiya chipfuyo chako chelethargic. - Izvi zvinonyanya kuenderana neiyoMultidrug resistance gene (MDR1)mumuviri wembwa.

Uyu "asingaoneki mutongi" wezvinodhaka metabolism akabata kiyi yekuchengetedzwa kwemishonga yemhuka dzinovaraidza, uyeMDR1 gene nucleic acid yekuongororandiyo nzira yakakosha yekuvhura iyi kodhi.

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Kiyi yeKuchengetedzwa Kwemishonga: Iyo MDR1 Gene

Kuti tinzwisise kukosha kweiyo MDR1 gene, tinofanira kutanga taziva "basa guru" rayo - kuita semushandi wekutakura wemetabolism yezvinodhaka. Iyo MDR1 gene inotungamirira kuumbwa kwechinhu chinonzi P-glycoprotein, iyo inonyanya kugoverwa pamusoro pemasero mumatumbo, chiropa, uye itsvo. Inoshanda sechiteshi chekutakura zvinodhaka chakatsaurirwa:

Mushure mokunge imbwa yatora mishonga, P-glycoprotein inoputira mishonga yakawandisa kubva mumasero uye inoadzinga kuburikidza netsvina kana weti, kudzivirira kuunganidza kunokuvadza mukati memuviri. Inodzivirirawo nhengo dzinokosha dzakadai seuropi nemwongo nekudzivirira kupinda kwezvinodhaka zvakanyanya izvo zvinogona kukonzera kukanganisa.

Nekudaro, kana iyo MDR1 gene ikachinja, uyu "mushandi wekutakura" anotanga kusashanda. Inogona kuita yakawandisa, kupomba zvinodhaka kunze nekukurumidza uye kukonzera kusakwana kwekuisa muropa, zvichideredza zvakanyanya kushanda kwezvinodhaka. Kana kuti inogona kunge yakakanganisa kushanda, kutadza kubvisa zvinodhaka munguva, zvichiita kuti mishonga iunganidze uye inokonzera migumisiro yakadai sekurutsa kana chiropa uye kukuvara kwetsvo.- Ichi ndicho chikonzero nei imbwa dzichigona kupindura zvakasiyana kune chaiyo chaiyo mishonga.

Zvakatonyanya kunetsandezvekuti zvikanganiso zveMDR1 zvinoita se "zvimbambaira" zvakavanzwa - kazhinji hazvionekwe kutozosvikira mushonga waunza njodzi. Somuenzaniso, dzimwe imbwa dzinoberekwa dziine magene akaremara MDR1, uye mwero wakajairwa wemishonga inorwisa utachiona (yakadai seivermectin) inogona kukonzera ataxia kana kuti koma painopihwa vachiri vadiki. Dzimwe imbwa dzine MDR1 yakawandisa dzingangonzwa kusununguka kunorwadza kubva kune maopioid kunyangwe kana ichidhawa chaiyo nehuremu. Matambudziko aya haakonzerwi ne“mishonga yakaipa” kana kuti “imbwa dzisingabatanidzi,” asi kuti pesvedzero yemajini.

Mukudzidzira kwekiriniki, mhuka dzinovaraidza dzakawanda dzinotambura zvakanyanya kutadza kweitsvo kana kukuvadzwa kwetsinga mushure mekutora mishonga pasina kutariswa kweMDR1 - zvisingatungamiriri kumitengo yepamusoro yekurapa asiwo kutambura kusiri madikanwa kumhuka.

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Genetic Testing yekudzivirira Njodzi dzeMishonga

Canine MDR1 gene nucleic acid yekuongorora ndiyo kiyi yekunzwisisa "chimiro chebasa" chemutakuri uyu pachine nguva. Kusiyana nechinyakare chekutarisa kutariswa kweropa - izvo zvinoda kudzokororwa ropa rinotorwa mushure memushonga - nzira iyi inoongorora zvakananga gene reMDR1 yembwa kuona kana shanduko iripo uye kuti ndeapi marudzi.

Pfungwa yacho iri nyore uye yakafanana neinokuvadza hyperthermia genetic test, ine matanho matatu makuru:

1. Sample Collection:

Nekuti iyo MDR1 gene iripo mumaseru ese, chete sampuli yeropa diki kana oral swab inodiwa.

2. Kutorwa kweDNA:

Murabhoritari inoshandisa mareagents akasarudzika kuparadzanisa DNA yembwa kubva mumuenzaniso, kubvisa mapuroteni uye kumwe kusvibiswa kuti iwane yakachena genetic template.

3. PCR Amplification uye Analysis:

Uchishandisa chaiwo probes akagadzirirwa akakosha MDR1 shanduko masaiti (seyakajairwa canine nt230 [del4] mutation), PCR inokudza chinongedzo chejini chimedu. Chiridzwa chacho chinobva chaona masaini efluorescent kubva kuprobe kuti aone mamiriro ekuchinja uye kushanda kwemaitiro.

Zvose zvinotora maawa anenge 1-3. Mhedzisiro yacho inopa nhungamiro yakananga kune veterinarians, zvichibvumira sarudzo dzakachengeteka uye dzakanyatsojeka dzemishonga pane kuvimba nekuedza-uye-kukanganisa.

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Innate Genetic Differences, Yakawanikwa Kuchengetedzwa Kwemishonga

Varidzi vezvipfuyo vangashamisika: Ko MDR1 abnormalities ndeyekuzvarwa here kana kuwanikwa?

Pane zvinhu zviviri zvakakosha, uye genetics iri yekutanga:

Breed-Specific Genetic Traits

Ichi ndicho chikonzero chinowanzoitika. Mari yekuchinja inosiyana zvakanyanya mumhando dzakasiyana:

Collies(kusanganisira Shetland Sheepdogs uye Border Collies) vane yakakwirira kwazvo nt230[del4] mutation rates - inenge 70% yepurebred Collies inotakura chirema ichi.
Vafudzi veAustraliauyeOld English Sheepdogsratidzawo mitengo yakakwira.
Inobereka zvakadaiChihuahuasuyePoodlesvane mwero wakaderera wekuchinja.

Izvi zvinoreva kuti kunyangwe imbwa isati yambotora mushonga, mhando dzine njodzi dzinogona kutakura shanduko.

Mishonga uye Kufurira Kwezvakatipoteredza

Nepo iyo MDR1 gene pachayo iri yekuzvarwa, kushandiswa kwenguva refu kana kurema kwemamwe mishonga kunogona "kumisikidza" abnormal gene expression.

Kushandiswa kwenguva refu kwezvimweantibiotics(semuenzaniso, tetracyclines) kanaimmunosuppressantsinogona kukonzera kubhadharwa kweMDR1, kutevedzera kuramba kwezvinodhaka kunyangwe pasina shanduko yechokwadi.

Mamwe makemikari ezvemamiriro ekunze (akadai sekuwedzera mune yakaderera-mhando zvigadzirwa zvemhuka) anogonawo kukanganisa zvisina kunanga kugadzikana kwemajini.

The MDR1 gene inokanganisa kuwanda kwemishonga, kusanganisira antiparasitic agents, painkillers, antibiotics, chemotherapy drugs, uye anti-epileptic mishonga. Semuyenzaniso:

A Collie akatakura chirema anogona kutambura zvakanyanya neurotoxicity kunyangwe kubva mukuteedza huwandu hweivermectin.

Imbwa dzine MDR1 yakawandisa dzingada kugadziridzwa madosi emishonga inorwisa fungal yezvirwere zveganda kuti ibudirire.

Ndosaka vanachiremba vemhuka vachisimbisa kutariswa kweMDR1 vasati vapa mishonga yemhando dzine njodzi.

Kune varidzi vezvipfuyo, MDR1 nucleic acid yekuyedza inopa dziviriro mbiri yekuchengetedza mishonga:

Kuongororwa kwembeu dzine njodzi kare kare (semuenzaniso, Collies) kunoratidza kupesana nemishonga kweupenyu hwose uye kunodzivirira kuchetura netsaona.

Imbwa dzinoda mishonga yenguva refu (seyekurwadziwa kusingaperi kana pfari) dzinogona kuve nemadosi akagadziridzwa.

Kuedza kununura kana imbwa dzakasanganiswa dzinobvisa kusava nechokwadi nezvengozi dzemajini.

Inonyanya kukosha kune imbwa dzechikuru kana avo vane zvirwere zvisingaperi, avo vanowanzoda mishonga.

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Kuziva Pamberi Zvinoreva Dziviriro Zvirinani

Zvichienderana nemhedzisiro yebvunzo, heano matatu ekuchengetedza mishonga kurudziro:

Mhuka dzine njodzi huru dzinofanirwa kukoshesa kuongororwa.

Collies, Australian Shepherds, nedzimwe mhando dzakafanana dzinofanira kupedza ongororo yeMDR1 isati yasvika mwedzi mitatu yezera uye chengeta zvabuda pafaira kuna chiremba wavo wemhuka.

Gara uchibvunza vet yako nezve "genetic kuenderana" usati wapa mushonga.

Izvi zvakakosha kune zvinodhaka zvine njodzi zvakanyanya senge antiparasitic mishonga uye painkillers. Kunyange kana rudzi rwembwa yako rusina ngozi, nhoroondo yekusagadzikana inoreva kuti kuongororwa kwemajini kunofanira kufungwa.

Dzivisa kuzvirapa nemishonga yakawanda.

Mishonga yakasiyana inogona kukwikwidza P-glycoprotein's transport channels. Kunyangwe zvakajairika MDR1 majini anogona kuremerwa, zvichikonzera kusaenzana kwemetabolism uye kuwedzera njodzi dzehupfu.

Ngozi yekuchinja kweMDR1 iri mukusaonekwa kwavo - kwakavanzwa mukati meiyo genetic kutevedzana, kusaratidza zviratidzo kudzamara mushonga watanga dambudziko.

Kuongororwa kweMDR1 nucleic acid inoita senge detector chaiyo yezvimbambaira, inotibatsira kunzwisisa maitiro embwa emushonga wemetabolism pachine nguva. Nekudzidza magadzirirwo ayo uye maitiro enhaka, kutanga kuongorora, uye kushandisa mishonga zvine hungwaru, tinogona kuona kuti kana mhuka dzedu dzinovaraidza dzichida kurapwa, dzinowana rubatsiro runoshanda apo dzichidzivirira njodzi dzemishonga - kuchengetedza hutano hwadzo nenzira ine mutoro.

Nguva yekutumira: Nov-20-2025