DNA methylation kuyedzwa yakasanganiswa ne smartphones yekutanga kuongororwa kwetumori uye leukemia kuongorora pamwe nehuremu hwe 90.0%!

Kuonekwa kwekutanga kwekenza kunoenderana nemvura biopsy ndiko kutungamira kutsva kwe cancer yekuona uye kuongororwa kweUS National Cancer Institute mumakore apfuura, nechinangwa chekuona kenza yekutanga kana kunyange pfungwa dzakakosha. Izvo zvakange zvashandiswa zvakanyanya sovel biomarker yekuongorora kwekutanga kwekuwoneka kwakasiyana-siyana, kusanganisira cancer yemapapu, gosta temple, gliomas uye glemocological tumors.

Iko kubuda kwemapuratifomu kuratidza methylation nyika (methylscape) biomars inokwanisa kuvandudza zvakanyanya kuongororwa kwekutanga kwegomarara, kuisa varwere pachiitiko chekutanga.

Nguva pfupi yadarika, vatsvagiri vakagadzira ruva rakareruka uye rakareruka renyama yekutsvagirwa kubva kuCysteamin Pakutanga kuongorora kuti Leukemia inogona kuitwa mukati memaminitsi gumi nemashanu mushure mekubuda kweDNA kubva pamuenzaniso weropa, nekurongeka kwe 90.0%. Chinyorwa chechinyorwa chakakurumidza kuona Cancer's cancer muropa revanhu uchishandisa Cysteamine-Yakachinjirwa Aunps uye muchina wokudzidza-unogonesa smartphone.

Mufananidzo 1. Kutsanya uye kutsanya kupfeka chikuva chekanasi kuongorora kuburikidza neCyst / Aunps zvikamu zvinogona kuitwa mumatanho maviri ari nyore.

Izvi zvinoratidzwa muhuwandu 1 Cyst / Aunps yakazowedzerwa kumhinduro yakasanganiswa. Zvakajairika uye zvakashata DNA ine dzakasiyana methylation zvivakwa, zvichikonzera zvigadziko zveDND zvine maitiro akasiyana-akasiyana. Zvakajairika DNA Aggregates zvakasununguka uye pakupedzisira maAggregates Cyst / AUNPS Mukupesana, iyo yakasarudzika methylation nhoroondo yeCancer DNA inotungamira kugadzirwa kwemasumbu makuru ezvimedu zveDNAST.

Mifananidzo ye96-tsime mahwendefa akatorwa achishandisa iyo smartpher camera. Cancer DNA yakayerwa neye smartphone yakaenzana nekudzidza kwemuchina uchienzaniswa neyekudzivirirwa-kwakavakirwa nzira.

Cancer kuongorora mune chaiyo yeropa samples

Kuti uwedzere kushandisirwa kweiyo Sensing Puratifomu, vaongorori vakashandisa sensor iyo yakabudirira kusiyanisa pakati peyakajairika uye canceus dna muropa reropa. Methylation mapatani kuCPG Sites Epigeneticy Regedza Gene Express. Muchinguva dzose dzeminamato marudzi, shanduko muDNAhyation uye nokudaro mukuratidzira kwemajini ayo anokurudzira Tumourigenesis akaonekwa achidzokorora.

Semhando yedzimwe cancers inosangana neDNAhycation, vaongorori vakashandisa samples dzeropa kubva kune varwere vane leukemia uye hutano hwakanaka kuti vaongorore kugona kweiyo methycation mamiriro ekusanganisa leukaemic cancers. Iyi methylation nyika biomarker kwete chete kunze kwekunze iripo nekukurumidza nzira dzekucherekedza, asi zvakare inoratidza kutaridzika kwekuwedzera kwekutanga kwekuonekwa kwehuwandu hwevanhu vakasarudzika vachishandisa iyi nyore uye yakatwasuka.

DNA kubva kuRopa Samples kubva kuna 31 Leukemia Varwere uye gumi nevaviri vane hutano vakaongororwa. Sezvinoratidzwa mubhokisi rebhokisi muhuwandu Izvi zvainyanya kukonzerwa neyakawedzerwa hydrophobicity inotungamira kuDense Aggregation yeCancer DNA, iyo yakadzivirira kuunganidzwa kweCyst / AUNPS. Nekuda kweizvozvo, izvi Nuoparticles zvakapararira zvakakwana mumatare ekunze egomarara, izvo zvakaguma nekupararira kwakasiyana kweCyst / AUNPS Adsorbed pane zvakajairika uye cancer dna Aggregates. ROC CURVES yakabva yaburitswa nekukasira chikumbaridzo kubva kune hushoma kukosha kwe ए

Mufananidzo 2. (a) Hunhu Hwakasimudza Tsika dzeCyst / Aunps Solutions dzinoratidza kuvapo kweyakajairwa (bhuruu) uye cancer (red) DNA pasi pemamiriro ezvinhu akagadziridzwa

(Da650 / 525) yebhokisi zvirongwa; (b) Kuongorora kweRoc uye kuongororwa kwemuedzo wekuongorora. . .

Sezvinoratidzwa mumufananidzo 2b, iyo nharaunda iri pasi peRoc Curve (AUC = 0.9274) yakawanikwa kune yakagadziriswa sensor yakaratidza yakanyanya kunzwa uye chokwadi. Sezvazvinogona kuoneka kubva kubhokisi renyaya, iyo yakaderera poindi inomiririra yakajairika dna yeDNA haina kunyatsoparadzaniswa kubva kune yakanyanya nzvimbo inomiririra cancer neCancer Brown; Naizvozvo, kudzoreredzwa kwehungwaru kwaishandiswa kusiyanisa pakati pezvakajairwa nekenza mapoka. Kupiwa seti yemasangano akazvimirira, inofungidzira mukana wechiitiko chinoitika, senge cancer kana yakajairika boka. Iyo inotsamira inoshanduka misana pakati pe0 uye 1. Mhedzisiro yacho saka mukana. Isu takasarudza mukana wekuzivisa kenza (p) zvichibva pane δA650 / 525 seinotevera.

uko b = 5.3533, w1 = -6.95. Kuti uwane sampuli yekuenzanisira, mukana weisingakwanisi kupfuura 0.5 zvinoratidza muenzaniso wakajairika, nepo mukana we0.5 kana kupfuura zvinoratidza chiratidzo che cancer. Mufananidzo 2C inoratidzira iyo matsrix inogadzirwa kubva pakubuda-iyo-yega yekuyambuka-yekushandiswa, iyo yakashandiswa kusimbisa kugadzikana kwenzira yekuenzanisa. Mufananidzo 2D kupfupisa iyo yekuongorora bvunzo yeiyo nzira, kusanganisira kunzwa, chokwadi, chakakodzera kukosha kukosha (PPV) kukosha kwakashata (NPV).

Smartphone-yakavakirwa biosensensors

Kuti uwedzere kurerutsa sampula yekuyera pasina kushandisa spectrophotometers, vaongorori vakashandisa manera ekungwarira (AI) kududzira ruvara rwemhinduro uye kusiyanisa pakati pevanhu vakawanda uye vane cance. Kupiwa izvi, chiratidzo chemakomputa chakashandiswa kushandura ruvara rweCyst / Aunps Solution muCyst / Curple) Kungwara kwehungwaru kunogona kuderedza mari uye kuvandudza kuwanikwa mukududzira ruvara rweNaneopartices mhinduro, uye pasina kushandisa chero chinhu chespcount hardphone smartphone. Pakupedzisira, marudzi maviri ekudzidza mwero, kusanganisira sango risina kurongeka (RF) uye rutsigiro vekret muchina (svm) dzakadzidziswa kuvaka mhando. Ose maviri eRF uye SVM Models akarongedza samples sezvinoita zvakanaka uye zvisina kunaka nekujeka kwe 90.0%. Izvi zvinoratidza kuti kushandiswa kwehunyanzvi hwekungwarira mufoni-mbozha-based biosensing zvinogoneka.

Mufananidzo 3. (a) Kirasi yeiyo mhinduro yakanyorwa panguva yekugadzirira kweiyo sampu yeiyo yemifananidzo inowana nhanho. (b) Mufananidzo wemienzaniso unotorwa panguva yemifananidzo kutora danho. .

Uchishandisa Cyst / Aunps, vatsvagiri zvakabudirira kukudziridza platform iri nyore yekuona methycape yekusaona yakajairika DNA kubva kuCancer Sampuli yeRoples yeLeukemia kuongorora. Iyo yakadzikiswa sensor yakaratidza kuti DNA yakabviswa kubva kune chaiyo ropa sampuli yakakwanisa nekukasira uye inobata-tsika varwere vane madiki mumaminitsi gumi nemashanu, uye vakaratidza chokwadi che9.3%. Kuti uwedzere kurerutsa muenzaniso kuyedzwa nekubvisa kudiwa kweiyo spectrophotometer, muchina wokudzidza wakasiyanisa ruvara rwepfungwa uye nekukasira vanhu vachishandisa mbozhanhare pikicha, uye nekurongeka kwaive zvakare kuchawanika na90.0%.

Reference: Doi: 10.1039 / D2RA05725E