DNA methylation yekuongorora yakasanganiswa nemafoni ekutanga kuongororwa kwemamota uye leukemia kuongorora nekunyatsoita 90.0%!

Kuonekwa kwegomarara kwekutanga kwakavakirwa pamvura biopsy inzira nyowani yekuonekwa kwegomarara uye kuongororwa kwakakurudzirwa neUS National Cancer Institute mumakore achangopfuura, nechinangwa chekuona kenza yekutanga kana maronda ekare. Yakave ichishandiswa zvakanyanya seyakajeka biomarker yekuongororwa kwekutanga kwezvirwere zvakasiyana siyana, kusanganisira kenza yemapapu, mamota emudumbu, gliomas uye gynecological tumours.

Kubuda kwemapuratifomu ekuona methylation landscape (Methylscape) biomarkers ine mukana wekuvandudza zvakanyanya iripo yekutanga kuongororwa kwegomarara, ichiisa varwere padanho rekutanga rinorapika.

Munguva pfupi yapfuura, vaongorori vakagadzira yakapfava uye yakananga sensing chikuva chemethylation landscape yekuona yakavakirwa pacysteamine yakashongedzwa goridhe nanoparticles (Cyst/AuNPs) yakasanganiswa ne-smartphone-based biosensor inogonesa kukurumidza kutarisisa kwekutanga kweakasiyana mabundu. Kuongororwa kwekutanga kwe leukemia kunogona kuitwa mukati memaminitsi gumi nemashanu mushure mekutorwa kweDNA kubva mumuenzaniso weropa, nekururama kwe90.0%. Musoro wenyaya ndeyeKukurumidza kuona cancer DNA muropa remunhu uchishandisa cysteamine-capped AuNPs uye muchina wekudzidza-inogonesa smartphone.

Mufananidzo 1. Chikuva chiri nyore uye chinokurumidza kuona chekuongorora kenza kuburikidza neCyst / AuNPs zvikamu zvinogona kuitwa mumatanho maviri ari nyore.

Izvi zvinoratidzwa muMufananidzo 1. Kutanga, mushonga wemvura wakashandiswa kuputsa zvidimbu zveDNA. Cyst/AuNPs dzakazowedzerwa kune yakasanganiswa mhinduro. Yakajairika uye yakashata DNA ine akasiyana methylation zvimiro, zvichikonzera zvidimbu zveDNA zvine maitiro akasiyana ekuzvibatanidza. Normal DNA aggregates zvakasununguka uye pakupedzisira aggregates Cyst/AuNPs, izvo zvinoita kuti red-shifted hunhu hweCyst/AuNPs, kuitira kuti kuchinja kweruvara kubva mutsvuku kusvika kupepuru kunogona kuonekwa neziso rakashama. Kusiyana neizvi, iyo yakasarudzika methylation mbiri yekenza DNA inotungamira mukugadzirwa kwemasumbu makuru ezvimedu zveDNA.

Mifananidzo ye96-tsime mahwendefa akatorwa uchishandisa smartphone kamera. Kanzera DNA yakayerwa ne smartphone ine muchina wekudzidza ichienzaniswa ne spectroscopy-yakavakirwa nzira.

Kuongororwa kenza mumasampuli eropa chaiwo

Kuwedzera kushandiswa kwepuratifomu yekunzwa, vaongorori vakaisa sensor yakabudirira kusiyanisa pakati peyakajairwa nekenza DNA mumasampuli eropa chaiwo. methylation mapatani paCpG nzvimbo epigenetically inoronga gene kutaura. Munenge marudzi ese egomarara, shanduko muDNA methylation uye nekudaro mukutaura kwemajini anokurudzira tumourigenesis akaonekwa achichinjana.

Semuenzaniso kune mamwe magomarara ane hukama neDNA methylation, vaongorori vakashandisa masampu eropa kubva kuvarwere veleukemia uye kudzora zvine hutano kuongorora kushanda kweiyo methylation landscape mukusiyanisa leukemic cancers. Iyi methylation landscape biomarker haisi kungopfuura nzira dziripo dzekukurumidza leukemia yekuongorora, asi zvakare inoratidza kugona kwekuwedzera kusvika pakuonekwa kwekutanga kwemhando dzakasiyana dzegomarara uchishandisa iyi bvunzo yakapusa uye yakatwasuka.

DNA kubva kumasampuli eropa kubva kuvarwere ve31 leukemia uye 12 vanhu vane hutano vakaongororwa. sezvinoratidzwa mubhokisi rebhokisi muMufananidzo 2a, kukanganiswa kwekenza yekenza (ΔA650 / 525) yakanga yakaderera kupfuura yeDNA kubva kumasampuli akajairwa. izvi zvainyanya kukonzerwa nekuwedzera hydrophobicity inotungamira kune dense kuunganidzwa kwegomarara DNA, iyo yakadzivirira kuunganidzwa kweCyst/AuNPs. Nekuda kweizvozvo, aya nanoparticles akaparadzirwa zvachose muzvikamu zvekunze zvegomarara, izvo zvakakonzera kupararira kwakasiyana kweCyst/AuNPs yakashambadzirwa pane yakajairwa uye cancer DNA aggregates. ROC curves yakabva yagadzirwa nekusiyanisa chikumbaridzo kubva pamutengo wakaderera we ΔA650 / 525 kusvika pamutengo mukuru.

Mufananidzo 2.(a) Hukama hwekunyudza kukosha kwecyst/AuNPs mhinduro dzinoratidza kuvepo kwezvakajairwa (blue) uye cancer (tsvuku) dna pasi pemamiriro akagadziridzwa.

(DA650/525) yezvirongwa zvebhokisi; (b) ROC kuongororwa uye kuongororwa kwekuongorora bvunzo. (c) Kuvhiringidzika matrix yekuongororwa kwevakajairwa uye varwere vegomarara. (d) Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) uye kunyatsoita nzira yakagadzirwa.

Sezvinoratidzwa muMufananidzo 2b, nzvimbo iri pasi peROC curve (AUC = 0.9274) yakawanikwa kune yakagadziridzwa sensor yakaratidza kunzwisiswa kwepamusoro uye zvakananga. Sezvinogona kuonekwa kubva mubhokisi rakarongeka, nzvimbo yakaderera inomiririra boka reDNA yakajairika haina kunyatsoparadzaniswa kubva panzvimbo yepamusoro inomiririra boka reDNA rekenza; saka, logistic regression yakashandiswa kusiyanisa pakati peyakajairika uye kenza mapoka. Zvichipihwa seti yezvakazvimiririra zvakasiyana, inofungidzira mukana wekuti chiitiko chiitike, senge gomarara kana boka rakajairwa. Iyo inotsamira yakasiyana inosiyana pakati pe 0 ne 1. Mhedzisiro naizvozvo inogoneka. Isu takasarudza mukana wekuzivikanwa kwekenza (P) zvichienderana neΔA650/525 sezvinotevera.

apo b=5.3533,w1=-6.965. Pakusarudzwa kwemuenzaniso, mukana weisingasviki 0.5 unoratidza sampuli yakajairika, nepo mukana we0.5 kana wepamusoro unoratidza sampu yegomarara. Mufananidzo 2c inoratidzira kuvhiringidzika matrix inogadzirwa kubva kune-yega-yoga-yakayambuka-yakagadziriswa, iyo yakashandiswa kusimbisa kugadzikana kwemaitiro ekugadzirisa. Mufananidzo 2d inopfupisa kuongororwa kwekuongorora kwekuongorora kweiyo nzira, kusanganisira sensitivity, chaiyo, yakanaka yekufungidzira kukosha (PPV) uye yakaipa yekufungidzira kukosha (NPV).

Smartphone-based biosensors

Kuenderera mberi kurerutsa kuyedzwa kwemuenzaniso pasina kushandisa spectrophotometers, vaongorori vakashandisa hungwaru hwekugadzira (AI) kududzira ruvara rwemhinduro uye kusiyanisa pakati pevanhu vakajairwa negomarara. Zvichipa izvi, kuona kwekombiyuta kwakashandiswa kushandura ruvara rweCyst / AuNPs mhinduro muDNA yakajairika (yepepuru) kana DNA ine kenza (tsvuku) uchishandisa mifananidzo ye96-tsime mahwendefa akatorwa kuburikidza nefoni kamera. Artificial intelligence inogona kuderedza mitengo uye kunatsiridza kuwanikwa mukududzira ruvara rwe nanoparticle mhinduro, uye pasina kushandisa chero optical hardware smartphone accessories. Pakupedzisira, mhando mbiri dzekudzidza muchina, dzinosanganisira Random Sango (RF) uye Tsigira Vector Machine (SVM) dzakadzidziswa kuvaka mhando. ese maRF neSVM modhi akanyatso rongedza masampuli seakanaka uye asina kunaka nekurongeka kwe90.0%. Izvi zvinoratidza kuti kushandiswa kwehungwaru hwekugadzira mufoni-based biosensing inogoneka.

Mufananidzo 3. (a) Target class yemhinduro yakanyorwa panguva yekugadzirira sampuli yedanho rekutora mufananidzo. (b) Muenzaniso mufananidzo wakatorwa panguva yekutora mufananidzo nhanho. (c) Kusimba kwemavara eiyo cyst/AuNPs mhinduro mune yega yega ye96-tsime ndiro yakatorwa kubva pamufananidzo (b).

Vachishandisa Cyst/AuNPs, vaongorori vakabudirira kugadzira yakareruka yekunzwa chikuva chekuona methylation landscape uye sensor inokwanisa kusiyanisa yakajairwa DNA kubva kune cancer DNA kana uchishandisa ropa chairo rekuongorora leukemia. Sensor yakagadziridzwa yakaratidza kuti DNA yakatorwa kubva muropa rechokwadi yakakwanisa kukurumidza uye inodhura-kuona zvishoma zvekenza DNA (3nM) muvarwere veleukemia mumaminitsi e15, uye yakaratidza chokwadi che95.3%. Kuwedzera kurerutsa kuyedzwa kwemuenzaniso nekubvisa kudiwa kweiyo spectrophotometer, kudzidza muchina kwakashandiswa kududzira ruvara rwemhinduro uye kusiyanisa vanhu vakajairwa negomarara vachishandisa mufananidzo wenharembozha, uye chokwadi chakakwanisa kuwanikwa pa90.0%.

Chirevo: DOI: 10.1039/d2ra05725e