Ongorora zvinowanika-shandura yedhiriji yekudzokorora mukutsvaga

Mafuta ekutenderera, anozivikanwawo sePRR michina, maturusi akakosha mumanyorero ebiology uye genetics yekutsvaga. Izvi zviridzwa zvinoshandiswa kuAmpliwy DNA uye rna kuburikidza nePolymerase Chain Reaction (PCR) tekinoroji. Nekudaro, iyo inosanganisira yedhorobhal cylecrs haina kungogumira kune pcr application. Munyaya ino, isu tichaongorora nzira dzakasiyana-siyana dzekutenderera dzinoshandiswa mukutsvaga nekukosha kwavo mukufambira mberi kwesainzi.

1. PCR org

Basa rekutanga rethermal cylerndeyekuita pcr imptizing, iyo inokosheswa kune akasiyana-siyana eiyo molecular biology application. Nekuisa Sampuli yeDNA yeDNA. Kuita uku kwakakosha kuongororwa kweGeneti, Gumi Expression zvidzidzo, uye kuwanikwa kwevamiririri vane hutachiona.

2. Quantitative PCR (QPCR)

Pamusoro peiyo standard pcr, yedhiramu cryclers inoshandiswa kune huwandu hwePCR kana QPCr, uchibvumira kuwanda kweiyo nyukireki acid zvinangwa mumuenzaniso. Nekubatanidza fluorescent dhayeti kana probes

3. Reverse transcriptptptupt

Dhizaini dzekutenderera dzinotamba basa rakakosha mune reverse transcriptptptptptptptptptptptptptptptptptptptptptptptptptptptptptptptptptptptpty Iyi nzira yakakosha yekufunda kuGene kutaura, rna hutachiona, uye MrNN inodhinda mapatani. Iyo yekudyara yedhiri ine chaiyo tembiricha yekudzora inokoshesa kubudirira kwert-pl-pcr miedzo.

4. Digital PCR

Kufambira mberi muThe Thermal Cyler Tekinoroji yakave nekuvandudza kwedhijitari pcr, inzira inotyisa kwazvo yehuwandu hwakakwana hwe acclic acids. Nekukamura prcr maitiro mune imwe yeMicroonaction

5. Kugadzirira kweinotevera-chizvarwa kutsakatika maraibhurari

Kufamba kweThelmal ndicho chikamu chakakosha chemubhadharo wekugadzirira maitiro ekutevera-kwechizvarwa kuteverwa (NGS) zvikumbiro. Nekuita PRR-yakavakirwa kuwedzera zvidimbu zveDND

6. Protein engineering uye Mutagesisis

Kuwedzera kune nucleic acid kukwikwidza, maTermal kutenderera anoshandiswa mune protein engineering uye mutagesesis fundo. Saiti-inotungamirwa Mutagensis, protein Expression Optimization, uye kutungamira kwekufunga kwekushanduka-shanduka kunowanzovimba nekudzora kwePRR.

7. Kugara kwezvakatipoteredza nekuchengetedza kwekuchengetedza

Mafuta ekutenderera anoshandiswawo mukuongororwa kwezvakatipoteredza uye zvekuchengetedza zvekuchengetedza PRR-yakavakirwa bvunzo dzinomhanya pane maTermy comedrs anogonesa nekukurumidza uye chaicho chiratidzo chekusvibisa, chengetedza kuchengetedzwa uye kunaka kwechikafu uye zvakatipoteredza samples.

Mukupfupikisa,theermal cyclersZvishandiso zvisinganzwisisike mumanyorero ebiology uye genetics research Kuita kwavo uye kunyatsoita kunoita kuti vatsvake pakuedza kubva kuGene Expression kuongororwa kune yekutarisisa kwemunharaunda. Sezvo tekinoroji inoenderera mberi, kutenderera kwemitambo kunogona kuita basa rinowedzera kukosha mukutyaira kwesainzi uye hunyanzvi.