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Kufambira mberi kwakabatana
Sekureva kweHofisi Yeruzivo yeBazi rezvekurima nenzvimbo dzekumaruwa, muna Nyamavhuvhu 2018, denda renguruve remuAfrica rakaitika muShenbei New District, Shenyang City, Liaoning Province, inova denda renguruve rekutanga muAfrica muChina. Kubva muna Ndira 14, 2019, denda renguruve reAfrica rakaitika mumatunhu anopfuura makumi maviri muChina, kuuraya nguruve 916000, zvichikonzera kushushikana neveruzhinji.
African swine fever (ASF)
ASF (African Swine fever) chirwere chakakomba chinotapukira chinokonzerwa neAfrica swine fever virus (ACFV) inobata nguruve dzepamba nenguruve dzomusango (African wild boar, European wild boar, etc.). Sangano repasi rose rehutano hwemhuka (OIE) rakachinyora sechirwere chemhuka chakataurwa zviri pamutemo, chinovawo mhando yedenda remhuka iro China rinotarisa kudzivirira.
Hunhu hwechirwere
Makiriniki anoratidza fivha (kusvika 40 ~ 42 ℃), tachycardia, dyspnea, chikamu chikosoro, serous kana mucinous purulent secretion mumaziso nemhino, ganda cyanosis, kubuda ropa kuri pachena kweitsvo, lymph node uye gastrointestinal mucosa. Zviratidzo zvekiriniki zveAfrica classical swine fever zvakafanana neye classical swine fever, iyo inogona kungoonekwa chete nekuongororwa murabhoritari.
Mhinduro kuAfrican classical swine fever
1. Sample processing
Inokodzera ropa uye matishu akasiyana evanofungidzirwa kuti vanorwara nguruve: spleen, lymph node uye itsvo tishu.
Mienzaniso yeropa
Tora 200 μ L sampuli yeropa, 5000g centrifugal 5 min, tora iyo supernatant kuti iongororwe.
Tissue samples
Mushure mekunge sampuli dzematishu dzaive dzakazara pasi, huwandu hwakakodzera hwesaline yakajairika kana PBS yakawedzerwa, uye iyo supernatant yaive centrifuged kuti iongororwe.
2. Kutora otomatiki
Hangzhou Bigfish Bio-tech Co., Ltd. BFEX-32 automatic nucleic acid extractor inogona kupedza kutorwa kwemasamples makumi matatu nemaviri mumaminitsi makumi matatu, ayo anogona kuzadzisa zvaunoda pakutora nhamba huru yemasampuli. Iyo yese maitiro haadi chero kumwe kushanda, inochengetedza nguva uye inoderedza chikanganiso chekushanda kwemanyorero, uye inovandudza kushanda kwekushanda zvakanyanya sezvinobvira.
3. Kuchena kwepamusoro nucleic acid kucheneswa
MagPure Nucleic acid yekubvisa kit, ine BFEX-32, chigadzirwa chakanyatsoenderana PCR & QPCR kuonekwa.
4. Kuongorora kwekombiyuta uye kuongorora
Zvinoenderana nemamiriro chaiwo erabhoritari, mushandisi anotora hunhu kana huwandu hwekuona chirongwa.
Hangzhou Bigfish Bio-tech Co., Ltd. inopa hunhu (Quantitative) kit yekuona yeAfrican classical swine fever (ACFV) ine FC-96G (BFQP-16/48), inogona kuona ACFV zvakanyanya, zvine hunyoro uye nekuvimbika.
Zvimwe zvirimo, ndapota teerera kune yepamutemo WeChat account yeHangzhou Bigfish Bio-tech Co., Ltd.
Nguva yekutumira: May-23-2021
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