Maitiro ekusarudza iyo chaiyo yekuraira yeTymal yezvido zvako zvekutsvaga

Theermal cyclersZvishandiso zvisingabvumirwe kana zvasvika kune molecular biology uye genetic tsvarhurumende. Zvakare inozivikanwa sePC (Polymerase Chain Reaction) muchina Nekudaro, pane zvakawanda zvingasarudzwa pamusika wekusarudza iyo chaiyo yekudyisa cyrerr yezvido zvako zvekutsvaga inogona kuve basa rinotyisa. Hezvino zvimwe zvinhu zvakakosha zvekufunga kana uchiita sarudzo yako.

1. Nzwisisa zvaunogona kutsvagisa zvinodiwa

Usati wadonha mune zvakatemwa zvevakasiyana denderedzwa kutenderera, zvakakosha kuti uongorore zvido zvako zvekutsvagisa. Funga nezverudzi rwekuedza iwe ruchaita. Uri kushandisa standard pcr, huwandu pccr (qpcr), kana yakakwira-yekubatira application? Imwe neimwe yezvikumbiro izvi ingangoda maitiro akasiyana uye kugona kweiyo yekudyara yedhiri.

2. Tembiricha yekudziya uye kusafanana

Iyo tembiricha yekudziya yeiyo yedhiri yedhisheni chinhu chinoshora. Vazhinji vePCR protocols vanowana dhizaini danho panosvika 94-98 ° C, nhanho inoshamisa pa50-65 ° C, uye nhanho yekuwedzera pa72 ° C. Ita shuwa kuti iyo yedhiri yemitambo yaunosarudza inogona kubata idzi tembiricha uye kuti tembiricha yakagoverwa zvakaringana mumufananidzo wese. Kufanana kwekushisa kwekushisa kunogona kukanganisa kutsvagurudza kwako nekukonzera mhedzisiro isingaenderane.

3. Block fomati uye kugona

Mafuta ekutenderera anouya mune dzakasiyana siyana maudular mafomu, kusanganisira 96-ell-ell-ell-ell malates, 384-tsime mahwendefa, uye kunyange 1536-tsime mahwendefa. Sarudzo yeBlock fomati inofanira kuenderana nezvinodiwa zvako. Kana iwe uri kuita yakakwira-yekupfuura kuedza, iwe ungangoda iyo yakakura block fomati. Kukurukurirana, kwekuedza zvidiki-zvidiki, ndiro 96-zvakanaka inogona kukwana. Pamusoro pezvo, funga kana iwe uchida manyoroshi ekuchinja mumatare akasiyana, sezvo izvi zvinogona kuwedzera kuwedzera kwekutsvaga kwako.

4. Kumhanya uye kushanda zvakanaka

Munzvimbo yanhasi yakamhan'arira yekutsvaga, nguva ndeye yakakosha. Tsvaga iyo yekudyara yedhorobha ine inokurumidza kupisa uye inotonhorera kugona. Mamwe marudzi epamberi anogona kuzadza plcr kutenderera mumaminitsi makumi matatu, achimhanyisa kukurudzira basa rako. Mukuwedzera, maficha akadai sekukurumidza maitiro kana kukurumidza kupisa mareti anowedzera kushanda zvakanaka, achikubvumira kuti uite mamwe ma samples munguva shoma.

5. Mushandisi interface uye software

Mushandisi-ane hushamwari interface yakakosha kuti ishandiswe inoshanda. Tarisa uone denderedzwa reziya neanosimudzira yekubata skrini, sarudzo dzakareruka programu, uye protocols protocols. Mhando yepamusoro dzinogona kuuya zvakare ne software iyo inobvumira kune chaiyo-nguva yekutarisa uye yekuongororwa kwedata, iyo inonyanya kubatsira kuti QPCER application. Ita shuwa kuti software inoenderana neyako iripo masisitimu uye inogona kubata iyo data yekubuda iwe yaunoda.

6. Budget Kufunga

Mafuta ekutuka anosiyana zvakanyanya mumutengo, saka zvakakosha kuve nebhajeti usati watanga kutenga imwe. Kunyange zvingave zvichinakidza kuenda nesarudzo yakachipa, funga kukosha kwenguva refu kwekudyara mumuchina wepamusoro-shinda unosangana nezvinodiwa zvako zvekutsvaga. Chimbofunga kwete chete mutengo wekutanga kutenga, asiwo mutengo wekutapudza, kugadzirisa, uye zvinogona kukwidziridzwa.

7. Mugadziri rutsigiro uye waranti

Pakupedzisira, funga nezve mwero wekutsigirwa uye waranti wakapihwa nemugadziri. Iyo yakavimbika yedhiri yemitambo inofanirwa kupa warnuived waranti uye uve nerutsigiro rwevatengi rwekugadzirisa dambudziko uye kugadzirisa. Izvi zvinokuponesa iwe nguva nezviwanikwa mukufamba kwenguva.

mumhedziso

Kusarudza kodzerothermal cylerKune zvaunoda zvekutsvagira isarudzo yakaoma inogona kukanganisa kubudirira kwekuedza kwako. Nekunyatsofunga nezvezvakakodzera zvaunoda, tembiricha renji, module fomati, kumhanya, mushandisi, uye mugadziri sarudzo, iwe unogona kuita sarudzo yekutsvagisa uye kuwana mhinduro dzako dzekutsvagisa uye uwane zvimwe zvakavimbika. Nguva yekudyara iri mune ino sarudzo yekusarudza inozopedzisira yabhadhara mumhando uye kushanda kwebasa rako resainzi.