Nzira yekusarudza iyo chaiyo yekupisa cycler kune zvaunoda pakutsvaga

Thermal cyclersmaturusi akakosha kana zvasvika kune molecular biology uye genetic research. Iyo inozivikanwawo sePCR (polymerase chain reaction) muchina, muchina uyu wakakosha pakukudza DNA, zvichiita kuti ive musimboti wemhando dzakasiyana dzemaapplication anosanganisira cloning, sequencing uye gene expression analysis. Nekudaro, pane zvakawanda zvingasarudzwa pamusika zvekuti kusarudza iyo chaiyo yekupisa cycler yezvido zvako zvekutsvagisa kunogona kuve basa rakaoma. Heano zvimwe zvakakosha zvekufunga nezvazvo pakuita sarudzo yako.

1. Nzwisisa zvaunoda pakutsvaga

Usati wanyura mune zvakatsanangurwa zveakasiyana anopisa cyclers, zvakakosha kuti uongorore zvaunoda chaizvo zvekutsvaga. Funga nezvemhando yekuedza yauchaita. Uri kushandisa yakajairika PCR, quantitative PCR (qPCR), kana yakakwira-kuburikidza application? Imwe neimwe yeaya maapplication angangoda akasiyana maficha uye kugona kweanopisa cycler.

2. Tembiricha Range uye Uniformity

Iyo tembiricha yemhando ye thermal cycler chinhu chakakosha. Zvizhinji zvePCR protocol zvinoda denaturation nhanho painoda kusvika 94-98°C, nhanho yekumisa pa50-65°C, uye danho rekuwedzera pa72°C. Ita shuwa kuti thermal cycler yaunosarudza inokwanisa kubata tembiricha idzi uye kuti tembiricha inogoverwa zvakaenzana mukati memodule. Kusafanana kwekushisa kwekushisa kunogona kukanganisa tsvagiridzo yako nekukonzera mhedzisiro isingaenderane.

3. Block fomati uye huwandu

Thermal cyclers inouya mumhando dzakasiyana dzemodular mafomati, anosanganisira 96-tsime mahwendefa, 384-tsime mahwendefa, uye kunyange 1536-tsime mahwendefa. Sarudzo yebhuroka fomati inofanirwa kuenderana nezvido zvako zvekufambisa. Kana iwe uri kuita zviyedzo zvepamusoro-soro, ungangoda yakakura block fomati. Sezvineiwo, kune zvidiki-zviyedzo, 96-tsime ndiro inogona kukwana. Pamusoro pezvo, funga kana iwe uchida anochinjika mamodule mumafomati akasiyana, sezvo izvi zvichigona kuwedzera kuita kwakasiyana-siyana kwekutsvagisa kwako.

4. Speed ​​​​uye Kubudirira

Munharaunda yemazuva ano yekutsvakurudza inokurumidza, nguva ndiyo inokosha. Tsvaga inodziya cycler ine kukurumidza kudziyisa uye kutonhora masimba. Mamwe mamodheru epamberi anogona kupedzisa PCR kutenderera mukati memaminitsi makumi matatu, achimhanyisa zvakanyanya kufamba kwako. Uye zvakare, maficha akadai sekukurumidza modhi kana kukurumidza kupisa mitengo inowedzera kushanda zvakanaka, zvichikutendera kuti ugadzirise mamwe masampuli munguva shoma.

5. User Interface uye Software

Mushandisi-ushamwari interface yakakosha pakushanda kwakanaka. Tsvaga inodziya cycler ine intuitive touch screen, yakapusa programming sarudzo, uye preset protocol. Mamodheru epamberi anogona kuuyawo nesoftware inobvumira kutarisisa-nguva chaiyo uye kuongororwa kwedata, iyo inonyanya kubatsira kune qPCR maapplication. Ita shuwa kuti software inoenderana neako aripo masisitimu uye inokwanisa kubata iyo data inobuda yaunoda.

6. Kufunga Kwebhajeti

Thermal cyclers inosiyana zvakanyanya mumutengo, saka zvakakosha kuve nebhajeti usati watanga kutenga imwe. Kunyangwe zvingave kuyedza kuenda nesarudzo yakachipa, funga kukosha kwenguva refu kwekudyara mumuchina wemhando yepamusoro unoenderana nezvido zvako zvekutsvagisa. Funga kwete chete mutengo wekutanga wekutenga, asiwo mutengo wezvekushandisa, kuchengetedza, uye zvinogona kukwidziridzwa.

7. Mugadziri Tsigiro uye Warranty

Pakupedzisira, funga nhanho yekutsigirwa uye waranti yakapihwa nemugadziri. Akavimbika anopisa cycler anofanirwa kupa waranti yakazara uye ave nerutsigiro rwevatengi rwekugadzirisa nekugadzirisa. Izvi zvinokuchengetera nguva uye zviwanikwa mukufamba kwenguva.

mukupedzisa

Kusarudza zvakanakathermal cyclernokuti zvaunoda tsvakurudzo isarudzo yakakosha inogona kukanganisa kubudirira kwekuedza kwako. Nekunyatso tarisa zvaunoda chaizvo, tembiricha yemhando, moduli fomati, kumhanya, mushandisi interface, bhajeti, uye nerutsigiro rwemugadziri, unogona kuita sarudzo ine ruzivo iyo inosimudzira kugona kwako kwekutsvagisa uye kuwana mhedzisiro yakavimbika. Kudyara nguva mune iyi sarudzo inozopedzisira yabhadhara mumhando uye kugona kwebasa rako resainzi.