Nat med | Iyo yakawanda-omtics nzira yekubuda iyo yakabatanidzwa bundu

Nat med | A akawanda-omtics nzira yekubuda kune iyo yekubatanidza iyo bundu rakabatanidzwa, immune yerudzi uye microbial nyika ye colorel cancer inoratidza kuwirirana kweiyo microbiome ine immune system
Kunyangwe biomarkers yegomarara repamusoro rakadzidziswa zvakanyanya mumakore apfuura, zvirongwa zvazvino zvekiriniki zvichingove nekugadziriswa kweDNA-microsate Vatsvagiri vakacherekedza kushamwaridzana pakati peGene Expression-yakavakirwa Immune Mhinduro, uye bundu remagetsi enzura

Sezvo kutsvagisa kwakafambira mberi, maitiro ezviuru ekarazi colorel cancer, kusanganisa cancer Colored, kusimuka,
Kubvisa hukama pakati pehukama hwechiitiko uye mhedzisiro, timu yevanoongorora kubva kuSilra Chikwata chakaitwa denderedzwa denderedzwa denderedzwa remhando yepamusoro kubva kune varwere vane makore makumi matatu nenhatu, vakadzika tucko rrna geti Chidzidzo ichi chakabudiswa mumushonga wechisikigo se "bundu rakabatanidzwa, immune immune uye microbiomome atlas ye cancer".

Chinyorwa chakaburitswa mumushonga wechisikigo

AC-ICAM Overview

Vanotsvagurudza vakashandisa denthogonal genomic chikuva chando kuti vatsvage tumor tumor tishu Zvichienderana neese-inonakidza Sequencing (Wes), rna-seq data control control, uye inosanganisira maitiro anobva kune varwere 340 vakachengetwa uye vanoshandiswa pakuongorora kuongororwa neyekutanga kutevera kwemakore kusvika makumi mana nemashanu. Chikwata chekutsvagisa chakatumidza izvi sosi sidra-lumc ac-icam: mepu uye kutungamira kune immune-cancer-cancer yekudyidzana (Mufananidzo 1).

Molecular Classification uchishandisa ICR

Kubata Modular Set of Immune genetic mamakisi eChincher Communollollollollowing ICR yakave yakabatanidzwawo nekupindirana mhinduro mumhinduro dzakasiyana-siyana dzemhando dzekenza, kusanganisira kenza yemazamu.

Kutanga, vatsvagiri vakasimbisa iyo ICR Signature yeiyo AC-ICM COHORT COHORT YAKABUDA MUZVINHU ZVINOGONESA Vatsvagiri vakaratidzira kuwanda kwenhandare inowirirana nemvumo yekubvumirana. Mapoka eCMS aisanganisira cms1 / immune, cms2 / canonical, cms3 / metabolic uye cms4 / mesenchymal. Kuongorora kwakaratidza kuti icr zviyero zvakarongeka zvine hungwaru nzira mune ese commy Subty Subty

Mune ese ma cms, huwandu hwechisikigo cheki (nk) cell uye t cell vakura muICR hights

Mufananidzo 1. AC-icam Kudzidza Dhizaini, Immune-inoenderana neGene Signature, immune immune uye molecular Subtypes uye kupona.
Icr inotora bundu-rakamiswa, rakamisirwa makasimudziro t masero
Vashoma chete masero eT masero anopinda muTumor tumor tishu rakanzi rive rakanangana neTumor vatorwa (pasingasviki 10%). Naizvozvo, ruzhinji rweIntra-tumor t masero anotaurwa seakasarudzika t masero (akabata masero t masero). Iko kuwirirana kwakasimba nehuwandu hwemasero emunharaunda maTCRS neTCRRS yakaonekwa muTcrs Scel Cell MuCheka Cluster (yakazara uye Cms Classification), iyo yakakwira clonality yeSecenal Incars yakaonekwa muCms uye CMME SUPTYPE (Mufananidzo 2C), ine chikamu chepamusoro cheICR-High Tumors. Uchishandisa mharidzo yese (1827 majini emajini), matanhatu icr gency Immunoseq TCR Clenality yakanangana zvakanyanya nemazhinji iCR majini pane kuora kunoonekwa uchishandisa Bundu-Inotaura CD8 + mamaki (Mufananidzo 2f uye 2G). Mukupedzisa, kuongorora pamusoro apa kunoratidza kuti iyo ICR Signature inobata kuvapo kwejora, yakafukidzwa misodzi maT masero uye anogona kutsanangura zvazvinoreva.

Mufananidzo 2. TCR Metrical uye Correlation ine majini ane chekuita nemidziyo, immune immune uye molecular subtypes.
Microbiomome kuumbwa mune hutano uye colon cancer tishu
Vaongorori vaitarisira 16s rrna sexequencing vachishandisa DNA yakabviswa kubva kuwiridziro yeDour uye ine hutano coloni kubva kune 246 varwere (Mufananidzo 3a). Zvekusimbiswa, vaongorori vanoongorora zvakare Kutanga, vatsvagiri vachienzanisa huwandu hwehukama hweFlora pakati pemataurirwo ematauriri uye ane hutano colon tishu. Clostrium Perfringeny yakawedzera zvakanyanya mu tumors ichienzaniswa nemhando dzehutano (Mufananidzo 3a-3D). Pakanga pasina mutsauko wakakosha muAlpha
Kuti uone kushamwaridzana kwakakodzera pakati pezvibodzwa zvemicrobial nepamapepa ezvipfuyo, vaongorori vane chinangwa chekushandisa 16s rrna gene geni yekuziva microbiome maficha anofanotaura kupukunyuka. AC-ICAM246, vatsvagiri vakamhanyisa os cox rex modhi iyo yakasarudzwa maficha makumi matatu nemasere macomputer's Njodzi), inonzi MBR Classifiers (Mufananidzo 3f).
Mune ino yekudzidziswa yekudzidzira (icam246), yakaderera mbr chikoro (mbr <0, Low mbr) yakabatana nenjodzi yakaderera yerufu (85%). Vanotsvagurudza vakasimbisa kushamwaridzana pakati pevhu rakapfuura mbr (njodzi) uye kwenguva refu os mune maviri akazvimiririra akasimbisa maCahorts (ICAM42 uye TCGA-Coad). .

Mufananidzo 3. Microbiome mune bundu uye hutano hwehuturu uye hukama neICR uye kuponeswa kwemurwere.
Mhedziso
Iyo yakawanda-omtics nzira inoshandiswa mune ino yekudzidza inogonesa kuona zvakakwana uye kuongororwa kwemanyorerwe emhinduro yekupindurwa kwemusoro muColeroc Cancer uye inoratidza kuwirirana pakati peMicrobiome uye Immune system. Yakadzika TCR Kuongorora kweTumor uye Hutano Hutano Rakaratidza kuti huwandu hwechiitiko cheICR zvinogona kunge zvichikonzerwa nekugona kwekutora bundu uye pamwe neTumor antigen-chaiyo t cell.

Nekuongorora tumor microbiomome kuumbwa uchishandisa 16s rrna gene geneequencing muAc-ICAM SAMPLES, iyo timu yakazivisa microbicate Kunyangwe siginecha iyi yakawanikwa kubva mumatumba ekutonga, pakanga paine kuwirirana kwakasimba pakati pehutano Nekubatanidza iyo icr uye mbr zviyero, zvaive zvinokwanisika kuziva uye kusimbisa mudzidzi wemhando yakawanda Iyo yekudzidza yakawanda-OMIC DATASET inopa sosi yekunzwisisa zviri nani colon cancer biology uye kubatsira kuwana zvakasarudzika mashandiro ekuratidzira nzira.

Reference:
Roelands, J., Kuppen, Pjk, Ahmed, Ei et Al. Iyo bundu rakabatanidzwa, immune immune uye microbiome auto of colon cancer. Nat med 29, 1273-1686 (2023).