Protocol yekuonekwa kwekwakabva mhuka yeBigfish

Dambudziko rekuchengetedza chikafu riri kuramba richikura. Sezvo musiyano wemutengo wenyama uri kuwedzera zvishoma nezvishoma, chiitiko che "kurembera musoro wemakwai uye kutengesa nyama yembwa" chinoitika kakawanda. Kufungidzirwa kubiridzira kwemanyepo uye kukanganisa kodzero nezvinodiwa nevatengi zviri pamutemo, zvinoderedza mukurumbira weveruzhinji wekuchengetedza chikafu, zvichikonzera kukanganisa munharaunda. Kuti tive nechokwadi chekuchengetedzwa kwechikafu uye kugadzirwa kwekuchengetedza zvipfuyo munyika yedu, zviyero zvekuongorora uye nzira dzinodiwa nekukurumidza.

Nekuenderera mberi kwekuvandudza uye kushingirira kwevatsvagiri, Bigfish yakazvimiririra yakagadzira iyo inotorwa nemhuka yekuona kititi, ichipa yakawedzera uye nekukurumidza mhinduro kune vatengi vedu! Isu tinonzwawo kukudzwa kukuru kukwanisa kubatsira vatengi vedu kugadzirisa matambudziko avo.
Zita rechigadzirwa: Mhuka yekuziva kit (nguruve, huku, bhiza, mombe, gwai)
Kunzwa kwakanyanya: zvishoma kuona muganho 0.1%
Hunhu hwepamusoro: kuzivikanwa kwakaringana kwemarudzi ese e "nyama chaiyo uye yenhema", hapana muchinjiko-reactivity
1, Sample processing
Mienzaniso yacho yakagezwa kaviri kusvika katatu ne70% ethanol uye mvura yakanyungudutswa kaviri, yakaunganidzwa mumachubhu akachena 50 ml centrifuge kana masaga akavharidzirwa akachena uye akachengetwa nechando pa -20 ° C. Mienzaniso yakakamurwa kuita zvikamu zvitatu zvakaenzana, kusanganisira sampu inoedzwa, sampu yakadzokororwa uye sampu yakachengetwa.
2, Nucleic acid kubviswa
Matishu emhando anoomeswa uye anogayiwa zvakanaka kana kuwedzerwa kune nitrogen yemvura, yobva yaiswa poda mudhaka uye pestle, uye mhuka genomic DNA inotorwa uchishandisa otomatiki.nucleic acid extractor + Magpure Animal Tissue Genomic DNA Kuchenesa Kit.

(Laboratory extraction set)

3. Amplification bvunzo
Muedzo wekusimudzira unoitwa pachishandiswa Bigfish sequential real-time quantitative fluorescence PCR analyzer + inotorwa nemhuka yekuona kit kuti inyatsoona kana nyama yacho yakasvibiswa zvinoenderana nemhedzisiro yakaipa, kuchengetedza zvirinani kodzero dzevatengi uye kuchengetedza chikafu.

Mienzaniso: Animal Origin Test Kit (Gwai)