Kuchinja PCR: FastCycler Thermal Cycler

Mumunda webiology yemamorekuru,ma "thermal cyclers" chishandiso chakakosha mukuita kwepolymerase chain reaction (PCR). Sezvo vaongorori nemarabhoritari vachitsvaga kushanda zvakanaka uye kururama, FastCycler yave chinhu chinochinja-chinja basa iri. Nehunyanzvi hwayo hwepamusoro uye kushanda kwayo kwepamusoro, FastCycler iri kuisa mwero mutsva wekupisa cycling.

FastCycler inofambiswa nezvinhu zvePeltier zvemhando yepamusoro kubva kuMarlow, USA. Zvinhu izvi zvakagadzirwa nokungwarira kuti zvipe kudzora tembiricha kwakanaka, nemazinga ekupisa anosvika 6 °C/s. Kugona uku kukurumidza kukwira kunoderedza zvikuru nguva inodiwa paPCR cycle yega yega, zvichibvumira vaongorori kupedzisa kuyedza nekukurumidza pasina kukanganisa kunaka.

Chimwe chezvinhu zvinoshamisa zveFastCycler ndechekuti inotenderera zvinhu zvinoshamisa, inodarika mamiriyoni zana emasekonzi. Kugara kwenguva refu uku kunoita kuti marabhoritari akwanise kushandisa FastCycler kwenguva yakareba, zvichiita kuti ive mari inodhura kune chero sangano rekutsvagisa. Kugara kwenguva refu kweFastCycler zvinoreva kuti vaongorori vanogona kutarisa pakuedza kwavo vasina kunetseka nezvekukundikana kwemidziyo kana kuda kutsiva nguva nenguva.

Kururama kwekupisa kwakakosha muPCR, uye FastCycler inobudirira panyaya iyi. Ichishandisa tekinoroji yepamusoro yekupisa nekutonhodza yethermoelectric, pamwe chete nePID (proportional-integral-derivative) tembiricha yekudzora, FastCycler inochengetedza mwero wepamusoro wekururama kwekupisa mukati menguva yese yekufamba nebhasikoro. Kururama uku kwakakosha kuti pave nemhedzisiro yakavimbika uye inodzokororwa, iyo inokosha mukutsvagisa kwesainzi.

Kufanana mumatsime ese chimwe chinhu chakakosha pakufamba kwemvura, uye FastCycler hainyadzisi. FastCycler yakagadzirirwa kuve nechokwadi chekuti tembiricha inofanana patsime rega rega, zvichideredza njodzi yekushanduka kwemhedzisiro yePCR. Kufanana uku kwakakosha zvikuru pakushanda nemasamples anoda kuwedzerwa kwakanyatsojeka, sezvo zvichibatsira kuona kuti maitiro ese anoitwa pasi pemamiriro akafanana.

Pamusoro pezvo, FastCycler inoshanda chinyararire, zvichiita kuti ive yakakodzera marabhoritari anoda nzvimbo yekushanda yakanyarara. Iyi nzira inobvumira vaongorori kuti vaite bvunzo vasina kukanganiswa neruzha rwemuchina, zvichigadzira mamiriro akatarisana uye ane zvibereko.

Kuwedzera kune hunyanzvi hwayo, FastCycler yakagadzirwa ichifunga nezvekushandisa kwayo zviri nyore. Inzwi rayo risinganzwisisike uye sarudzo dziri nyore dzekuronga mapurogiramu zvinoita kuti zvive nyore kune vese vaongorori vane ruzivo uye vatsva kushandisa. Kugona kugadzirisa maprotocol uye kutarisa kufambira mberi kuri nyore kunoita kuti ruzivo rwemushandisi wese ruwedzere, zvichibvumira masayendisiti kutarisa pakutsvaga kwavo pane kushandisa muchina wakaoma.

Muchidimbu, FastCyclerThermal Cyclerinomiririra kufambira mberi kukuru mutekinoroji yePCR. Nezvinhu zvayo zvePeltier zvemhando yepamusoro, kumhanya nekukurumidza, cycling index yakanaka, uye kudzora tembiricha yepamusoro, inopa mashandiro asina kuenzana ekushandisa molecular biology. Kusanganiswa kwekururama, kufanana, uye kushanda kweruzha rushoma kunoita kuti FastCycler ive chishandiso chakakosha kune chero rabhoritari inovavarira kuwana mhedzisiro yepamusoro zvinobudirira. Sezvo kudiwa kwePCR nekukurumidza uye kwakavimbika kuri kuramba kuchikura, FastCycler inomira semutungamiriri mubasa iri, ichigonesa vaongorori kusundira miganhu yekuwana kwesainzi.