Summer Science Guide: Kana 40°C Heat Wave Inosangana NemaMolecular Experiments

Kupisa kwakanyanya kwakaramba kuchiitika munzvimbo zhinji dzeChina nguva pfupi yadarika. Musi waChikunguru 24, Shandong Provincial Meteorological Observatory yakapa yambiro yeyero tembiricha yepamusoro, ichifanotaura tembiricha "sauna-yakafanana" ye35-37 ° C (111-133 ° F) uye 80% mwando kwemazuva mana anotevera munzvimbo dziri mukati menyika. Tembiricha munzvimbo dzakaita seTurpan, Xinjiang, dzava kusvika 48°C (111-133°F). Wuhan neXiaogan, Hubei, vari pasi peyambiro yeorenji, netembiricha inodarika 37°C mune dzimwe nzvimbo. Mukupisa uku kunopisa, nyika ine microscopic pasi pepamusoro pepipettes inosangana nekuvhiringidzika kusingawanzoitiki-kugadzikana kwe nucleic acids, kushanda kwema enzymes, uye chimiro chemuviri we reagents zvose zvinyararire zvakakanganiswa neheatwave.

Nucleic acid kubviswa kwave kukwikwidzana nenguva. Kana tembiricha yekunze ikadarika 40°C, kunyangwe paine air conditioner yakabatidzwa, tembiricha yetafura yekushanda inowanzokwira pamusoro pe28°C. Panguva ino, sampuli dzeRNA dzakasiiwa munzvimbo yakavhurika dzinoshatisa kanopfuura kaviri nekukurumidza semuchirimo uye matsutso. Mune magineti bead kudhirowa, iyo buffer mhinduro inozadzwa munharaunda nekuda kwekukurumidzira kuvhuvhuta kweiyo solvent, uye makristasi anonaya zviri nyore. Aya makristasi anozokonzera shanduko huru mukuita kweiyo nucleic acid kutora. Kusagadzikana kweorganic solvents kunowedzera panguva imwe chete. Pa30°C, huwandu hwechloroform volatilization hunowedzera ne40% zvichienzaniswa ne25°C. Panguva yekushanda, zvinodikanwa kuve nechokwadi kuti kumhanya kwemhepo mufume hood ndeye 0.5m / s, uye kushandisa magirovhosi e nitrile kuchengetedza kushanda kwekudzivirira.

Miedzo yePCR inotarisana nekukanganisa kwakanyanya kwekushisa. Mareagents akadai seTaq enzyme uye reverse transcriptase anonzwa zvakanyanya nekumwe kushanduka kwekushisa. Condensation pamadziro echubhu mushure mekubviswa kubva pa -20 ° C mufiriji inogona kukonzera pamusoro pe15% kurasikirwa kwekuita kwe enzyme kana ikapinda mumaitiro ekuita. dNTP mhinduro dzinogonawo kuratidza kudzikira kunooneka mushure memaminitsi mashanu chete ekuratidzwa tembiricha yekamuri (> 30°C). Kushanda kwezviridzwa kunokanganiswawo nekupisa kwakanyanya. Kana tembiricha yemurabhoritari iri> 35 ° C uye kupisa kwekubvisa kwePCR chiridzwa kusingakwane (<50 cm kubva kumadziro), musiyano wekushisa wemukati unogona kusvika kusvika 0.8 ° C. Kutsauka uku kunogona kukonzera kukwidziridzwa kumucheto kwe96-tsime ndiro kudonha nepamusoro pe40%. Mafirita eguruva anofanirwa kucheneswa nguva dzose (kuunganidza guruva kunoderedza kupisa kwekupisa ne50%), uye yakananga air conditioning inofanira kudziviswa. Uyezve, kana uchiita PCR kuyedza husiku, dzivirira kushandisa PCR chiridzwa se "makeshift firiji" kuchengetedza samples. Kuchengetedza pa4 ° C kweanopfuura maawa maviri kunogona kukonzera condensation kuti iumbe mushure mokunge chivharo chinopisa chavhara, kuderedza maitiro ekugadzirisa uye zvinogona kukanganisa ma modules esimbi.

Takatarisana nekunyeverwa kwekushisa kwepamusoro-soro, marabhoritari emamorekuru anofanirawo kuridza alarm. Akakosha eRNA samples anofanirwa kuchengetwa kumashure kwe -80°C firiza, iine mukana wekupinda panguva yekupisa kwepamusoro. Kuvhura gonhi re -20°C firiza kanopfuura kashanu pazuva kuchawedzera kushanduka kwetembiricha. Midziyo inogadzira kupisa-yakanyanya inoda inosvika masendimita makumi mashanu enzvimbo yekupisa kupisa kumativi ese uye kumashure. Uyezve, zvinokurudzirwa kugadzirisa nguva yekuedza: 7:00-10:00 AM yekushanda-kunzwa kwekushisa kwakadai seRNA kubvisa uye qPCR kurodha; 1:00-4:00 PM yebasa risiri rekuedza rakadai sekuongorora data. Iri zano rinogona kudzivirira zvinobudirira kukwirira kwepamusoro-soro kubva pakukanganisa matanho akakosha.

Kuedza kwema molecular panguva yeheatwave muedzo wezvose unyanzvi uye mwoyo murefu. Pasi pezuva rezhizha risingaregi, pamwe yave nguva yekuisa pasi pipette yako uye kuwedzera imwe bhokisi rechando kumasampuli ako kubvumira chiridzwa kuti chibvise kupisa kwakawanda. Uku kuremekedzwa kwekushanduka kwetembiricha ndiko kunonyanya kukosha murabhoritari mukati memwedzi yechirimo inopisa—pamusoro pazvo, mukupisa kwe40°C kwechirimo, kunyange mamorekuru anoda “nzvimbo yepolar” yakanyatsochengetedzwa.