Gwaro Rinokosha reMachubhu ePCR ane Zvidimbu 8: Kuchinja Kufamba Kwebasa Renyu muLab

Munyaya yebiology yemamorekuru, kunyatsojeka uye kushanda zvakanaka zvakakosha zvikuru. Chimwe chishandiso chinovandudza zvakanyanya mashandiro emurabhoritari ichubhu yePCR ye8-plex. Aya machubhu matsva akagadzirirwa kurerutsa maitiro epolymerase chain reaction (PCR), zvichibvumira vaongorori kuita kuyedza zviri nyore uye nemazvo. Mublog rino, tichaongorora mabhenefiti emachubhu ePCR e8-plex, mamiriro ekushandisa, uye mazano ekuti vangawedzera sei kugona kwavo murabhoritari.

Chii chinonzi machubhu ePCR ane strip 8?

Machubhu ePCR ane zvidimbu zvisereYakagadzirwa nemachubhu masere akasiyana ePCR akabatanidzwa mumutsara kuti agadzire chubhu ine strip. Dhizaini iyi inobvumira kuti sampuli dzakawanda dziwedzere panguva imwe chete, zvichiita kuti ive yakakodzera kuyedza kwepamusoro-soro. Chubhu yega yega yePCR iri muchubhu ine strip inogona kutakura huwandu hwakati hwemusanganiswa we reaction, kazhinji 0.1 ml kusvika 0.2 ml, iyo yakakodzera kushandiswa kwakasiyana-siyana kwePCR.

Mabhenefiti ekushandisa machubhu ePCR ane zvidimbu zvisere

1. Kuvandudza mashandiro: Chimwe chezvakanakira zvikuru kushandisa machubhu ePCR ane zvidimbu zvisere kuchengetedza nguva yekugadzirira sampuro. Panzvimbo pekubata machubhu ePCR ega ega, vaongorori vanogona kurodha sampuro dzakawanda panguva imwe chete, zvichideredza njodzi yekusvibiswa uye kukanganisa kwevanhu.
2. Zvisingadhuri uye zvinobudirira: Nekushandisa tambo dzekuedza, marabhoritari anogona kuderedza huwandu hwezvinhu zvinodiwa pakuedza. Izvi hazvingoderedzi mari chete, asi zvinoderedzawo kukanganiswa kwepurasitiki inoraswa pane zvakatipoteredza.
3. Kutevera sampuro kwakavandudzwa: Machubhu mazhinji ePCR ane zvidimbu zvisere anouya nenzvimbo dzakanyorwa zvakajeka, zvichibvumira vaongorori kuona sampuli zviri nyore. Chinhu ichi chakakosha mukuyedza uko kuteverwa kwesampuli kwakarurama kwakakosha kuti kuve nechokwadi chekuti bvunzo dzinodzokororwa.
4. Inoenderana nekushandisa otomatiki: Sezvo marabhoritari ari kuramba achishandisa tekinoroji yekuzvigadzira, dhizaini yechubhu yePCR ine zvidimbu zvisere inoenderanawo nemasisitimu ekuzvigadzira. Kuenderana uku kunowedzera simba rekushandisa uye kunotsigira magadzirirwo akaomarara ekuyedza.
5. Kuchinja-chinja: Machubhu ePCR ane zvidimbu zvisere anogona kushandiswa mumabasa akasiyana-siyana, anosanganisira quantitative PCR (qPCR), reverse transcription PCR (RT-PCR), uye genotyping. Kushandiswa kwayo kwakasiyana-siyana kunoita kuti ive chishandiso chakakosha mumarabhoritari mazhinji ebiology.

Kushandiswa kwechubhu yePCR ine strip 8

Mashandisirwo emachubhu ePCR ane zvidimbu zvisere akasiyana-siyana. Anowanzo shandiswa pa:

• Kuongorora chirwere: Mumarabhoritari ekurapa, machubhu ePCR ane zvidimbu zvisere anogona kushandiswa kuona zvirwere zvinotapukira, zvirwere zvemajini, uye zviratidzo zvegomarara nekukurumidza.
• Tsvagiridzo nekuvandudzwa: Munzvimbo dzekutsvakurudza kwedzidzo neindasitiri, machubhu aya anobatsira pakutsvaga majini, kugadzira majekiseni, uye mamwe mashandisirwo ebiology yemamorekuru.
• Sainzi yezvekuferefeta: Kukwanisa kuwedzera DNA kubva muzviyero zvidiki kunoita kuti machubhu ePCR ane mitsetse misere ave akakosha mukuferefeta kwe forensic, uko humbowo hwese hunokosha.

Mazano ekushandisa zvakanyanya machubhu ePCR ane strip 8

1. Gadzirisa mamiriro ekuita: Iva nechokwadi chekuti mamiriro ePCR akagadziridzwa kuti aenderane nekuyedza kwako. Izvi zvinosanganisira kugadzirisa tembiricha yekunyungudutsa, nguva yekuwedzera, uye huwandu hweenzyme.
2. Shandisa ma reagent emhando yepamusoro: Kubudirira kwePCR kunoenderana zvikuru nemhando ye reagents dzinoshandiswa. Kungosarudza DNA polymerase yemhando yepamusoro, ma primer, uye ma buffers chete ndiko kunogona kuwanikwa mhedzisiro yakavimbika.
3. Chengetedza kusabereka: Kuti udzivise kusvibiswa, shandisa nzira yekusauraya utachiona nguva dzose paunenge uchibata machubhu ePCR ane zvidimbu zvisere. Izvi zvinosanganisira kupfeka magirovhosi, kushanda munzvimbo yakachena, uye kudzivirira kusvibiswa pakati pemasampuli.
4. Kuchengetedza kwakakodzera: Chengeta machubhu ePCR asina kushandiswa ane zvidimbu zvisere munzvimbo inotonhorera, yakaoma kuti arambe akasimba. Tevedzera nhungamiro dzekuchengetedza dzemugadziri.

mukupedzisa

Machubhu ePCR ane zvidimbu zviseretekinoroji inovhiringidza mumunda we molecular biology, ine mabhenefiti akawanda anogona kuvandudza kushanda zvakanaka kwemurabhoritari uye kururama. Nekunzwisisa mabhenefiti ayo nemashandisirwo ayo, vaongorori vanogona kushandisa maturusi aya kuvandudza mashandiro ebasa uye kuwana mhedzisiro yakavimbika. Kunyangwe uri kuita diagnostics yekiriniki, tsvakiridzo yesainzi kana ongororo ye forensic, kuisa machubhu ePCR ane 8-strip murabhoritari yako kunogona kuvandudza kushanda zvakanaka kwebasa rako. Gamuchira ramangwana rePCR uye tarisa kuedza kwako kuchibudirira nemhinduro iyi itsva!