Basa reiyo chaiyo-nguva PCR masisitimu mune yakasarudzika mushonga uye genomics

Real-time PCR (polymerase chain reaction) masisitimu ave maturusi akakosha muminda inokurumidza kubuda yemishonga yakasarudzika uye genomics. Aya masisitimu anogonesa vaongorori nevarapi kuti vaongorore genetic zvinhu nekurongeka kusati kwamboitika uye nekukurumidza, vachigadzira nzira yekugadzira nzira dzekurapa dzemunhu uye kuwedzera kunzwisisa kwezvirwere zvakaomarara.

Real-nguva PCR masisitimu, inozivikanwawo sehuwandu hwePCR (qPCR), panguva imwe chete kukudza uye kuenzanisa DNA kana RNA mumuenzaniso. Tekinoroji iyi inonyanya kukosha mukurapa kwemunhu, uko marapiro anoenderana nemasikirwo emunhu. Nekupa chaiwo zviyero zvemajini ekutaura mazinga, chaiyo-nguva PCR masisitimu anobatsira kuona biomarker inogona kufanotaura mhinduro yemurwere kune chaiyo kurapa. Mune oncology, semuenzaniso, mazinga ekutaura emamwe majini anogona kuratidza kana murwere angangobatsirwa neanonangwa marapirwo, nekudaro achitungamira sarudzo dzekurapa nekuvandudza mhedzisiro.

Pamusoro pezvo, chaiyo-nguva PCR masisitimu anoita basa rakakosha mumunda we genomics, kwaanogona kushandiswa kusimbisa zvakawanikwa kubva kumusoro-kuburikidza kutevedzana matekinoroji. Nepo kutevedzana kwechizvarwa chinotevera (NGS) kuchigona kupa tarisiro yakazara yegenome remunhu, chaiyo-nguva PCR inogona kusimbisa kuvepo uye huwandu hweakasiyana mageneti akaonekwa kuburikidza nekutevedzana. Uku kusimbiswa kwakakosha kuve nechokwadi chekuvimbika kwe data ye genomic, kunyanya mumakiriniki ekugadziriswa uko sarudzo dzinoitwa zvichienderana neruzivo rwemajini.

Iko kuita kwakasiyana-siyana kweiyo chaiyo-nguva PCR masisitimu haina kuganhurirwa kune oncology uye genomics. Izvo zvinoshandiswawo mukuongororwa kwechirwere chinotapukira, uko nekukurumidza uye kwakaringana kuona kwehutachiona kwakakosha. Semuenzaniso, panguva yeCOVID-19 denda, chaiyo-nguva PCR yakava chiyero chegoridhe chekuongorora chirwere cheSARS-CoV-2. Kugona kuyera huwandu hwehutachiwana hwemurwere kwete chete rubatsiro mukuongororwa, asi zvakare hunogona kuzivisa nzira dzekurapa uye mhinduro dzehutano hweveruzhinji.

Pamusoro pekuongororwa, chaiyo-nguva PCR masisitimu anogona zvakare kubatsira kutarisa kufambira mberi kwechirwere uye kushanda kwekurapa. Nekuyera shanduko mukutaura kwemajini nekufamba kwenguva, varapi vanogona kuongorora kuti murwere ari kupindura sei kurapwa. Uku kutarisisa kwakasimba kunonyanya kukosha kuzvirwere zvisingaperi, sezvo marapirwo ekurapa angangoda kugadziridzwa zvichienderana nekuchinja kwemajini emurwere.

Sezvo tekinoroji ichifambira mberi, kubatanidzwa kweiyo-chaiyo-nguva PCR masisitimu mumishonga yemunhu uye genomics kwakawedzera kuvandudzwa. Masisitimu emazuva ano ari kuwedzera mushandisi-ane hushamwari, ane otomatiki maficha anokwenenzvera mafambiro uye kuderedza mukana wekukanganisa kwevanhu. Mukuwedzera, kukurudzirwa kwe multiplex real-time PCR inobvumira kuonekwa panguva imwe chete yezvinangwa zvakawanda mukuita kumwe chete, kuwedzera zvakanyanya kuburikidza nekubudirira.