Izvo zvakasiyana-siyana uye kukosha kwemahwendefa akadzika-tsime murabhoritari yemazuva ano

Munyika inogara ichishanduka yekutsvagisa nekuyedza kwesainzi, maturusi nemidziyo inoshandiswa mumarabhoritari inoita basa rakakosha mukubudirira kwemapurojekiti akasiyana. Chimwe chishandiso chakakosha kudaro ndiro rakadzika-tsime. Aya mahwendefa ane hunyanzvi ave anofanirwa-kuve nawo mumarabhoritari mazhinji, kunyanya muminda yakaita semolecular biology, biochemistry, uye kuwanikwa kwezvinodhaka. Mune ino blog, isu tichaongorora mashandiro uye kukosha kwemahwendefa akadzika-mutsime, mashandisiro awo, uye zvakanakira zvavanounza kune vanotsvaga.

Chii chinonzi tsime rakadzika ndiro?

A ndiro yakadzika tsimeimicroplate ine nhevedzano yematsime, rimwe nerimwe rakagadzirirwa kuchengeta mavhoriyamu makuru emvura pane yakajairwa microplate. Mahwendefa akadzika emutsime anowanzo gadzirwa emhando yepamusoro epurasitiki uye anouya mune akasiyana masisitimu ane tsime masimba kubva pa1 ml kusvika pa50 ml kana kupfuura. Aya mahwendefa akagadzirirwa kubvumira kuchengetedza kwakanaka kwemuenzaniso, kusanganisa, uye kuongorora, zvichivaita chishandiso chakakosha mune dzakawanda marabhoritari marongero.

Kushandiswa kwendiro yetsime rakadzika

Mahwendefa etsime rakadzika ane huwandu hwakawanda hwekushandisa, kusanganisira asi kwete kugumira ku:

1. Sample Storage: Vatsvakurudzi vanowanzoshandisa mahwendefa emvura yakadzika kuchengetedza kwenguva refu yezvinyorwa zvipenyu zvakadai seDNA, RNA, mapuroteni, uye tsika dzemasero. Iyo yakakura simba retsime, iyo yakachengeteka sampu inogona kuchengetwa pasina njodzi yekubuda kana kusvibiswa.
2. Kuongorora kwepamusoro-soro: Mukutsvaga zvinodhaka uye kubudirira, mahwendefa akadzika-tsime anokosha kune yepamusoro-soro yekuongorora (HTS) maitiro. Vanoita kuti vaongorori vaedze zviuru zvemakomboni panguva imwe chete, zvichikurumidzisa kuzivikanwa kwevanogona kukwikwidza zvinodhaka.
3. PCR uye qPCR: Mahwendefa etsime rakadzika anowanzo shandiswa polymerase chain reaction (PCR) uye quantitative PCR (qPCR) application. Iwo akagadzirirwa kugonesa kufambisa bhasikoro uye kuderedza njodzi yekusvibiswa-pakati pakati pemasampuli.
4. Protein crystallization: Muchimiro chebiology, mahwendefa akadzika-tsime anoshandiswa pakuedza kweprotein crystallization. Iwo maburi akakura anopa nzvimbo yakakwana yekukura kwekristaro, iyo yakakosha pazvidzidzo zveX-ray crystallography.
5. Sero Culture: Mahwendefa akadzika tsime anoshandiswawo kutsika masero munzvimbo inodzorwa. Dhizaini yavo inobvumira mitsara yesero yakawanda kuti igadziriswe panguva imwe chete, ichifambisa zvidzidzo zvekufananidza uye zviedzo.

Zvakanakira kushandisa tsime rakadzika ndiro

Kushandisa mahwendefa akadzika tsime kunopa akati wandei mabhenefiti ayo anowedzera kushanda nesimba uye kurongeka murabhoritari yako:

1. Kuwedzera Kugona: Mukana mukuru wemapureti etsime rakadzika kugona kwavo kubata mavhoriyamu akakura emvura, iyo inonyanya kubatsira pakuedza inoda huwandu hukuru hwesample.
2. Deredza njodzi yekusvibiswa: Dhizaini yendiro yakadzika-tsime inoderedza njodzi yekusvibiswa pakati pemasampuli uye inovimbisa kuvimbika kwemhedzisiro yekuedza.
3. Kuenderana neAutomation: Mapuleti mazhinji akadzika-mutsime anowirirana neautomated fluid kubata masisitimu, achigonesa yakakwirira-kuburikidza workflows uye kuderedza mukana wekukanganisa kwevanhu.
4. Zvishandiso Zvakasiyana-siyana: Sezvambotaurwa, mabhodhoro akadzika anogona kushandiswa mumhando dzakasiyana dzekushandisa, achiaita chishandiso chinogoneka chevatsvaguri pamatunhu akawanda.
5. Mari-inoshanda: Nekugadzirisa akawanda masampuli panguva imwe chete, mahwendefa akadzika-tsime anogona kuchengetedza nguva uye zviwanikwa, pakupedzisira kuchengetedza mari yekushandisa marabhoritari.

mukupedzisa

Mukupedzisa,ndiro dzakadzika dzetsimechikamu chakakosha chemaitiro emazuva ano marabhoritari. Kuita kwavo kwakasiyana-siyana, kuwedzera kugona, uye kuenderana neotomatiki kunoita kuti ive chishandiso chakakosha kune vanotsvaga munzvimbo dzakasiyana siyana. Sezvo tsvakiridzo yesainzi irikuenderera mberi, kukosha kwemapureti akadzika ematsime kunongokura, zvichigadzira nzira yezvitsva zvakawanikwa uye zvitsva. Kunyangwe iwe uri kuita zvekuwanikwa kwezvinodhaka, molecular biology, kana chero imwe nzira yesainzi, kuisa mari mumapureti akadzika emhando yepamusoro kunogona kuwedzera zvakanyanya kugona kwako kutsvaga.