Kuvhura zvakavanzika zvehupenyu: kukosha kwe nucleic acid yekubvisa kits

Mumunda wemanyorerwo ebialogy, kuongororwa kweNyclic acids Nucleic acid yekubvisa kits yakatsauka maitiro aya, ichiita kuti ive inoshanda, yakavimbika, uye inowanikwa kune vanotsvaga uye vatengesi pasi rese. Mune ino blog, isu tichaongorora kits iyi, kukosha kwezvavakaita, uye kukanganisika kwavo pane kufambisira mberi kwesainzi.

Chii chinonzi nucleic acid yekubvisa kit?

Nucleic acid yekubvisa kitsZvishandiso zvakanyatsogadzirirwa kupatsanura DNA kana rna kubva kune akasiyana siyana ebhaibheri emasampulogy, senge ropa, tishu, masero, uye kunyange samples. Iyi kits inowanzove neese reagents uye protocol inodiwa kuti ifambise maitiro ekubvisa, kuona vaongorori vanogona kuwana yakakwirira-mhando nucleic acids neisiki kusvibiswa.

Maitiro ekutsamwa

Maitiro ekubvisa kazhinji anosanganisira nzira dzinoverengeka dzinokosha: Cell Lynis, kucheneswa, uye exution.

Cell Lynis: Iyo yekutanga nhanho ndeyekuvhura masero kuti abudise nuclelic acids. Izvi zvinowanzoitwa uchishandisa lyiss buffer ine magetsi uye enzymes ayo anovhiringidza masero menduru uye pureturu dzenongedzo.

Kunatswa: Mushure mekunge nuclec acids inoburitswa, iyo inotevera nhanho ndeye kubvisa zvinongedzo senge mapuroteni, lipids, uye mimwe marara emusha. Makiti mazhinji anoshandisa silica yeSilica kana magineti emagiresi kuti asarudze acids Acids, nekushambidza kusvibiswa.

Elution: Pakupedzisira, iyo yekunyepedzera nyuclic acids yakanatswa mune yakakodzera buffer, yakagadzirira kudzora maficha akadai sePCr, kutsakatika, kana kuvhara.

Nei uchishandisa nuclec acid yekubvisa kit?

Kubudirira: Tsika Nucleic acid Actid yekubvisa nzira dziri kupedza nguva uye kushanda-zvakanyanya. Nucleic acid yekubvisa kits kurerutsa maitiro uye inogona kuwanzo zadza kubviswa mukati meawa.

Kuenderana: Iyo yakagadziriswa protocol yakapihwa neiyi kits inochengetedza kubereka uye kuvimbika kwemhinduro. Izvi zvakakosha pakuedza uko kurongeka kwakaoma, senge clinical diagnostics kana kutsvagisa.

Kuedza Kunyangwe iwe uri kushanda nema samples yevanhu, chirimwa tishu, kana tsika dzezvemicrobial, panogona kunge paine kit kuti ienderane nezvaunoda.

Ushamwari Hushamwari: Mazhinji midziyo acidid yekubvisa midziyo inouya nemirairo yakazara uye yakagadzirirwa kuve nyore kushandisa, kunyangwe kune avo vangave vasina ruzivo rwebharabheni. Izvi zvakavezwa mwero mukana wekuti mendekopecial biologes maitiro, uchibvumira vaongorori vatambi kuti vatore chikamu mukutsvaga genetic.

Kushandiswa kwe nucleic acid yekubvisa

Iyo nuclec acids inowanikwa kubva kune iyi kits inogona kushandira sehwaro hwekushandisa zvikumbiro zvakawanda:

Gumi Ratsotsvaga: Kunzwisisa Gene Basa, kutaura uye mutemo.
Clinical diagnostics: Kuonekwa kwezvirwere zve genetic, chirwere chinotapukira nekenza.
Maonero eSainzi: Kuongororwa kweDNA sampuli yekuongorora kwematsotsi.
Bikechnology yeBiotechnology: Iko kuvandudzwa kwezvinhu zvakagadziridzwa zvakagadziridzwa (GMOS) kuwedzera goho rizi.
mumhedziso
Nucleic acid yekubvisa kitsZvishandiso zvisinganzwisisike mumasokisi emazuva ano mauraoloji, achibvumira vaongorori kuti vasunungure zvakavanzika zvehupenyu kune moleky alwe. Kubudirira kwavo, kusanzwisisika, uye kupesana nekushandura mamiriro ekutsvaga genetic uye diagnostics, zvichiita kuti zvive nyore kupfuura zvambosha kuti uongorore kuoma kweDNA uye rna. Sezvo tekinoroji inoramba ichisimudzira, tinogona kutarisira kuti kits idzi dzienderere mberi, tichivhura magonhi matsva kune kuwanikwa kwesainzi uye ruzivo. Kunyangwe iwe uri muongorori ane ruzivo kana mutsva kumunda, kuisa mari mumhando yemhando yekubvumidzwa kit inogona kuvandudza kunaka kwebasa rako uye mupiro mukuziva kweruzivo mune genetics.