Kusiyana-siyana kweDeep Well Plates muLabhoritari Research

Mahwendefa etsime rakadzikachinhu chikuru mukutsvagisa marabhoritari, chinopa mhinduro dzakasiyana uye dzinoshanda kune dzakasiyana siyana dzekushandisa. Aya mahwendefa e-multiwell akagadzirirwa kugarisa masampuli nenzira yepamusoro-soro, achiaita chishandiso chakakosha muzvidzidzo zvakasiyana zvesainzi zvakaita segenomics, proteomics, kuwanikwa kwezvinodhaka, nezvimwe.

Imwe yemabhenefiti makuru emaplateti etsime rakadzika kugona kwavo kubata mavhoriyamu makuru emasampuli. Aya mahwendefa ane kudzika kwakadzika kubva pa2 kusvika pa5 mm uye anogona kutora mavhoriyamu ekuenzanisa kusvika ku2 ml patsime, achiaita akanakira maapplication anoda kugadziriswa kwemavhoriyamu makuru emasampuli. Ichi chimiro chinonyanya kukosha mu-high-throughput screening assays apo akawanda masampuli anoda kugadziriswa panguva imwe chete.

Kuwedzera kune yakakwira sampuli kugona, mahwendefa akadzika matsime anowirirana neakasiyana ekushandisa marabhoritari, anosanganisira otomatiki emvura ekubata masisitimu, centrifuges, uye maplate readers. Kuenderana uku kunobvumira kusanganisa kusingaite mune iripo marabhoritari workflows, kugadzirisa maitiro uye kuwedzera kugona. Ingave yakashandiswa kugadzirira semuenzaniso, kuchengetedza kana kuongorora, mahwendefa akadzika etsime anopa yakavimbika uye iri nyore chikuva chekuitisa bvunzo.

Mukuwedzera, mahwendefa akadzika-tsime anowanikwa mumhando dzakasiyana-siyana, kusanganisira 96-, 384-, uye 1536-magadzirirwo ematsime, achipa vatsvakurudzi nekushanduka-shanduka zvichienderana nezvido zvavo zvekuedza. Uku kuchinjika kunoita mahwendefa akadzika-tsime akakodzera kune zvakasiyana-siyana zvekushandisa, kubva musero tsika uye microbial kukura kusvika kuprotein crystallization uye komputa kuongorora.

Dhizaini yemapuleti akadzika etsime zvakare inoita kuti ive yakanaka kune sampuli yekuchengetedza uye kuchengetedza. Kuvaka kwavo kwakasimba uye kuenderana nesarudzo dzekuisa chisimbiso dzakadai semafirimu anonamira uye magasi evhavha anovimbisa kutendeseka kwemuenzaniso uye kuderedza njodzi yekusvibiswa. Izvi zvinogadzira mahwendefa akadzika matsime akanakira kuchengetwa kwenguva refu kwemasampuri ezvipenyu, mareagents nemakomponi, zvichipa vaongorori mhinduro yakavimbika yekutarisira sampuli.

Pamusoro pezvo, mahwendefa akadzika etsime anowanikwa mune zvakasiyana-siyana zvezvinhu, zvinosanganisira polypropylene uye polystyrene, imwe neimwe ine yakasarudzika mabhenefiti zvichienderana nekushandisa. Semuenzaniso, mahwendefa e-polypropylene akadzika-tsime anozivikanwa nekupikisa kwemakemikari uye kuwirirana nemhando dzakasiyana-siyana dzekunyungudutsa, zvichiita kuti dzive dzakakodzera kushandiswa kunosanganisira makemikari ane utsinye. Polystyrene yakadzika-tsime mahwendefa, kune rumwe rutivi, anowanzo kufarirwa kujeka kwawo kwemaziso, achiaita akanakira maapplication anoda kutariswa kwekuona kana kuonekwa kwefluorescence.

Muchidimbu,ndiro dzakadzika dzetsimechishandiso chakakosha mukutsvagisa marabhoritari, chinopa kuita kwakasiyana-siyana, kushanda nesimba, uye kuvimbika mune zvakasiyana siyana zvekushandisa. Yavo yepamusoro sampuli kugona, kuenderana nerabhoritari chiridzwa, uye kuchinjika mumafomati uye zvigadzirwa zvinovaita zvinhu zvakakosha kune vanoongorora munzvimbo dzakasiyana dzesainzi. Ingave ndeyekugadziriswa kwemuenzaniso, kuchengetedza kana kuongorora, mahwendefa akadzika matsime anoramba achiita basa rakakosha mukusimudzira kuwanikwa kwesainzi uye hunyanzvi.