Kushandiswa Kwakawanda Kwemapuratifomu Akadzama Mukutsvaga muRabhoritari

Mapureti emvura yakadzikainyaya huru mukutsvagisa murabhoritari, ichipa mhinduro dzinoshanda uye dzinoshanda dzemashandisirwo akasiyana-siyana. Maplate aya e multiwell akagadzirwa kuti agone kugamuchira masampuli nenzira yepamusoro, zvichiita kuti ave chishandiso chakakosha muzvidzidzo zvakasiyana-siyana zvesainzi zvakaita se genomics, proteomics, drug discovery, nezvimwewo.

Chimwe chezvakanakira zvikuru zvemapureti emvura yakadzika kugona kwawo kubata huwandu hwakawanda hwemasampuli. Mapureti aya ane kudzika kwemvura kubva pa2 kusvika ku5 mm uye anogona kugamuchira huwandu hwemasampuli hunosvika 2 ml patsime, zvichiita kuti ave akakodzera kushandiswa kunoda kugadziriswa kwehuwandu hwakawanda hwemasampuli. Chinhu ichi chinonyanya kukosha mukuyedza kuongorora kwakawanda uko masampuli akawanda anofanirwa kugadziriswa panguva imwe chete.

Pamusoro pekukwanisa kwayo kuburitsa sampuro, maplate emvura yakadzika anoenderana nemidziyo yakasiyana-siyana yekurabhoritari, kusanganisira masystem ekubata mvura otomatiki, macentrifuge, uye maplate readers. Kuenderana uku kunobvumira kubatanidzwa zvakanaka mumaworkflows aripo erabhoritari, kugadzirisa maitiro uye kuwedzera kushanda zvakanaka. Ingave ichishandiswa pakugadzirira sampuro, kuchengetera kana kuongorora, maplate emvura yakadzika anopa puratifomu yakavimbika uye iri nyore yekuita kuyedza.

Pamusoro pezvo, maplate emvura yakadzika anowanikwa mumhando dzakasiyana-siyana, kusanganisira 96-, 384-, uye 1536-well configurations, zvichipa vaongorori mukana wekuchinja zvichienderana nezvavanoda pakuedza. Kuchinjika uku kunoita kuti maplate emvura yakadzika akwanise kushandiswa zvakasiyana-siyana, kubva pakukura kwemasero uye kukura kwehutachiona kusvika pakugadzirwa kwemapuroteni nemakristaro uye kuongorora zvinhu zvakasiyana-siyana.

Kugadzirwa kwemapureti emvura yakadzika kunoitawo kuti akwanise kuchengetwa nekuchengetwa kwemasampuli. Kugadzirwa kwawo kwakasimba uye kuenderana kwawo nesarudzo dzekusunga dzakadai semafirimu anonamira uye magasket echivharo zvinovimbisa kunaka kwemuenzaniso uye zvinoderedza njodzi yekusvibiswa. Izvi zvinoita kuti mapureti emvura yakadzika akwanise kuchengetwa kwenguva refu kwemasampuli ehupenyu, ma reagents uye mishonga, zvichipa vaongorori mhinduro yakavimbika yekugadzirisa mienzaniso.

Pamusoro pezvo, mapurati emvura yakadzika anowanikwa mumhando dzakasiyana-siyana dzezvinhu, kusanganisira polypropylene nepolystyrene, imwe neimwe ine zvayakanakira zvakasiyana zvichienderana nekushandiswa kwayo. Semuenzaniso, mapurati emvura yakadzika epolypropylene anozivikanwa nekudzivirira makemikari uye kushanda kwawo nemakemikari akasiyana-siyana, zvichiita kuti akwanise kushandiswa nemakemikari akaomarara. Mapurati emvura yakadzika epolystyrene, kune rumwe rutivi, anowanzo kufarirwa nekuda kwekujeka kwawo kwemaziso, zvichiita kuti ave akakodzera kushandiswa kunoda kuongororwa nemaziso kana kuonekwa kwechiedza chechiedza.

Muchidimbu,matura akadzika etsimechishandiso chakakosha mukutsvagisa murabhoritari, zvichipa kushanduka-shanduka, kushanda zvakanaka, uye kuvimbika mumhando dzakasiyana dzemashandisirwo. Kugona kwavo sampuli, kuenderana nemidziyo yerabhoritari, uye kuchinjika mumafomu nezvinhu zvinoita kuti zvive zvinhu zvinokosha kune vaongorori muminda yakasiyana-siyana yesainzi. Ingave yekugadzirisa, kuchengetera kana kuongorora mienzaniso, maplate emvura yakadzika anoramba achiita basa rakakosha mukusimudzira kuwanikwa kwesainzi nehunyanzvi.