Ndeipi nzira yekuparadzanisa nekuchenesa nucleic acids?

Nucleic acids, kusanganisira DNA neRNA, ma biomolecule akakosha anoita basa rakakosha muma genetics, molecular biology, uye biotechnology. Kukwanisa kuparadzanisa nekuchenesa ma nucleic acids aya kwakakosha pakushandiswa kwakasiyana-siyana, kusanganisira cloning, sequencing, uye gene expression analysis. Nucleic acid purification systems inosanganisira nzira dzakasiyana-siyana dzakagadzirirwa kubvisa nekuchenesa ma nucleic acids kubva mu biological samples. Chinyorwa chino chinoongorora nzira dzekuparadzanisa nekuchenesa ma nucleic acids uye chinosimbisa kukosha kwema systems aya mukutsvagisa kwesainzi yemazuva ano.

Kunzwisisa kucheneswa kwe nucleic acid

Kucheneswa kweNucleic acid kunoreva kubviswa kweDNA kana RNA kubva mumasero kana munyama, zvichiteverwa nekubvisa tsvina dzakadai semapuroteni, mafuta, nedzimwe tsvina dzemasero. Kuchena uye kuvimbika kwe nucleic acids dzakaparadzana kwakakosha pakushandiswa kwepasi, sezvo tsvina inogona kudzivirira enzyme reactions uye kukanganisa kururama kwemhedzisiro yekuedza.

Nzira dzakajairika dzekuparadzanisa uye kuchenesa nucleic acid

Kubviswa kwePhenol-chloroform:Nzira iyi yechinyakare inoshandisa zvinonyungudutsa zve organic kuparadzanisa nucleic acids kubva kumapuroteni nezvimwe zvikamu zvemasero. Muenzaniso wacho unosanganiswa ne phenol ne chloroform, zvichiita kuti nucleic acids iparadzane kuita aqueous phase, nepo mapuroteni achiramba ari mu organic phase. Mushure mekutenderera, aqueous phase ine nucleic acids inounganidzwa uye inosanganiswa ne ethanol.

Nzira dzekushandisa gel yeSilica:Mamembrane eSilica gel anoshandiswa zvakanyanya mumakit ekuchenesa nucleic acid ekutengesa. Chinangwa cheiyi nzira ndechekuti nucleic acids isunge kune silica gel ine munyu wakawanda. Mushure mekusunga, tsvina inobviswa, uye nucleic acids inobviswa nechinhu chine munyu mushoma kana mvura. Iyi nzira inodiwa nekuti inokurumidza, inoshanda, uye inoburitsa nucleic acids dzakachena zvakanyanya.

Kucheneswa kwemagineti:Nzira iyi inoshandisa mabhora emagineti akaputirwa ne nucleic acid binding agents. Kana sampuro ikasanganiswa nemabhora emagineti, mabhora emagineti anonamira pamusoro pemabhora. Mabhora acho anozoparadzaniswa nemushonga uchishandisa magineti, nokudaro achibvisa tsvina. Nzira iyi inogona kushandiswa otomatiki, zvichiita kuti ive yakakodzera kushandiswa kwemagetsi akawanda.

Chromatography yekoramu:Nzira iyi inosanganisira kupfuudza sampuro nepakoramu yechromatographic yakazara nechikamu chisingachinji chinochengetedza nucleic acids nenzira yakasarudzika. Mhando dzakasiyana dzemakoramu dzinogona kushandiswa, kusanganisira idzo dzakavakirwa pakubvisa saizi kana misimboti yekuchinjana kwemaion. Nucleic acids dzinobuda mukoramu, zvichikonzera sampuro yakacheneswa.

Nzira dzeEnzymatic:Nzira dzeEnzymatic, dzakadai sedzinoshandisa DNase kana RNase, dzinogona kushandiswa kubvisa nucleic acids kana zvinhu zvinosvibisa zvisingadiwe. Nzira iyi inonyanya kushanda pakugadzira sampuro dzakaoma, dzakadai sedzine DNA neRNA.

Mukupedzisa

Kupatsanurwa uye kucheneswa kweNucleic acid matanho akakosha mukutsvaga nekushandisa mamorekuru ebiology.Masisitimu ekuchenesa nucleic acidkupa vaongorori nzira dzakasiyana-siyana dzekuwana nucleic acids dzemhando yepamusoro dzakakodzera kushandiswa pasi pemvura. Ingave kushandisa nzira dzechinyakare dzekubvisa phenol-chloroform kana nzira dzemazuva ano dzakadai se silica gel kana magnetic bead-based cleansing, sarudzo yenzira inoenderana nezvinodiwa chaizvo zvekuedza uye rudzi rwemuenzaniso. Nekufambira mberi kwetekinoroji, masisitimu aya ekuchenesa akaramba achichinja, achivandudza kushanda, kumhanya, uye kuvimbika, pakupedzisira zvichivandudza kugona kwevaongorori mumunda we molecular biology.