Kiti yekuchenesa DNA yeMagaPure Blood Genomic

Tsananguro pfupi:

Kit iyi ine superparamagnetic microspheres uye premade extraction buffer, uye yakakodzera kuburitsa genomic DNA kubva muropa idzva, rakaomeswa nechando, uye rakachengetedzwa kwenguva refu. Zvidimbu zve genomic DNA zvakakura, zvakachena kwazvo, uye zvine hunhu hwakagadzikana uye hwakavimbika. DNA yakabviswa yakakodzera kuyedza kwakasiyana-siyana kwakadai sekugaya enzyme, PCR, kuvaka raibhurari, Southern hybridization, uye high-throughput sequencing.

TAURA NESU

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Zvimiro zvechigadzirwa

Mhando dzakasiyana dzemashandisirwo emuenzaniso:DNA ye genomic inogona kutorwa zvakananga kubva mumienzaniso yakadai seropa risingaunganidzi ropa (EDTA, heparin, nezvimwewo), buffy coat, uye blood clots.
Kurumidza uye nyore:Kuongororwa kwemuenzaniso uye kubatana kwe nucleic acid zvinoitwa panguva imwe chete. Mushure mekuiswa kwemuenzaniso pamuchina, kuburitswa kwe nucleic acid kunoitwa otomatiki, uye genomic DNA yemhando yepamusoro inogona kuwanikwa mumaminitsi anopfuura makumi maviri.
Yakachengeteka uye haina chepfu:Reagent iyi haina zvinhu zvinonyungudutsa zvine chepfu zvakaita sephenol nechloroform, uye ine simba guru rekuchengetedza.

Zvishandiso zvinochinjika

Hove hombe BFEX-32E/BFEX-32/BFEX-96E

Magadzirirwo ehunyanzvi

Huwandu hwemuenzaniso:200μL
Kuwanikwa kweDNA:≧4μg
Kuchena kweDNA:A260/280≧1.75

Tsanangudzo

Zita rechigadzirwa	Katsi. Kwete.	Kurongedza
Kiti yekuchenesa DNA yeMagaPure Blood Genomic （Pakeji yakazadzwa kare）	BFMP02R	32T
Kiti yekuchenesa DNA yeMagaPure Blood Genomic （Pakeji yakazadzwa kare）	BFMP02R1	40T
Kiti yekuchenesa DNA yeMagaPure Blood Genomic （Pakeji yakazadzwa kare）	BFMP02R96	96T