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Ivhu, senzvimbo yakasiyana-siyana yezvakatipoteredza, rine tupukanana twakawanda, kusanganisira mhando dzakasiyana-siyana dzetupukanana twakadai semabhakitiriya, fungi, mavhairasi, cyanobacteria, actinomycetes, protozoa uye nematodes. Dziine mabasa akasiyana-siyana ekugadzirisa muviri uye hunhu hwemuviri, dzinoita basa rakakosha mukutenderera kwezvinovaka muviri muvhu uye dzakakosha pakubvisa tsvina yevhu. Seimwe yenzvimbo dzakasiyana-siyana dzehupenyu pasi rose, zvidzidzo zve molecular biology zvevhu zvine kukosha kwakasiyana kwehupenyu. Mukuita uku, kuwana microbial DNA kubva muvhu ndiyo nhanho yekutanga mukutsvaga ivhu uye ndiyo nhanho yakakosha yekubudirira kwekuyedza kwemvura iri pasi. Zvisinei, pamusoro petupukanana twakawanda, ivhu rinowanzova nehuwandu hwakawanda hwemetabolites (humic acid, xanthic acid nezvimwe zvinhu zvehumic), izvo zvinogona kucheneswa zviri nyore pamwe chete ne nucleic acids panguva yekuburitsa nucleic acid, zvichikanganisa PCR iri pasi uye maitiro ekutevera.Hove HuruSequencing Soil and Faecal Genomic DNA Purification Kit inogona kuburitsa DNA yakachena uye yakabatana zvakanyanya kubva mumasampuli ane humus yakawanda setsvina yevhu, iyo inobatsira zvikuru pakutsvaga kusiyana kwezvipembenene zvevhu.
Chigadzirwa cheHove Huru
Chigadzirwa ichi chinoshandisa sisitimu yakasiyana yebuffer yakagadzirwa uye yakagadziriswa uye ine magineti inosunga DNA, iyo inogona kusunga nekusveta, kuparadzanisa nekuchenesa nucleic acids nekukurumidza, zvichiita kuti ive yakakodzera kupatsanurwa nekukurumidza uye zvinobudirira kweDNA ye genomic kubva muvhu netsvina, pamwe nekubvisa zvasara zvakaita se humic acids, mapuroteni, salt ions, nezvimwe zvasara. Kuenzanisa neBeaglefly sequencing magnetic bead method nucleic acid extractor, yakakodzera kwazvo kuburitsa otomatiki saizi yakakura yesampuro. DNA ye genomic yakabviswa yakachena uye ine mhando yepamusoro uye inogona kushandiswa zvakanyanya muPCR/qPCR, NGS nedzimwe tsvakiridzo dzekuedza.
Zvinhu zvirimo
Hunhu hwakanaka:kuparadzaniswa uye kucheneswa kweDNA ye genomic, goho guru, kuchena kwakanaka.
Mhando dzakasiyana-siyana dzemienzaniso:inogona kushandiswa zvakanyanya mumhando dzese dzevhu netsvina.
Kurumidza uye nyore:kuburitsa otomatiki uchishandisa chinoburitsa chinoenderana nacho, chakakodzera zvikuru kuburitsa mienzaniso mikuru.
Yakachengeteka uye haina chepfu:hapana chikonzero chekushandisa zvinhu zvine chepfu zvakaita sephenol/chloroform, nezvimwewo.
Zvishandiso zvinogona kugadziriswa:BFEX-32/ BFEX-32E/ BFEX-96E
Nguva yekutumira: Chikunguru-10-2025
Zvirongwa zvekuchengetedza ruzivo